Changes in the chemical barrier composition of tears in Alzheimer's disease reveal potential tear diagnostic biomarkers

Gergo Kalló, M. Emri, Zsófia Varga, Bernadett Ujhelyi, J. Tőzsér, Adrienne Csutak, Éva Csosz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is one of the most common neurodegenerative diseases, with increasing prevalence affecting millions of people worldwide. Currently, only autopsy is able to confirm the diagnosis with a 100% certainty, therefore, biomarkers from body fluids obtained by non-invasive means provide an attractive alternative for the diagnosis of Alzheimer's disease. Global changes of the protein profile were examined by quantitative proteomics; firstly, electrophoresis and LC-MS/MS were used, thereafter, SRM-based targeted proteomics method was developed and applied to examine quantitative changes of tear proteins. Alterations in the tear flow rate, total tear protein concentration and composition of the chemical barrier specific to AD were demonstrated, and the combination of lipocalin-1, dermcidin, lysozyme-C and lacritin was shown to be a potential biomarker, with an 81% sensitivity and 77% specificity.

Original languageEnglish
Article numbere0158000
JournalPLoS One
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Biomarkers
Alzheimer disease
Tears
biomarkers
Alzheimer Disease
Proteomics
proteomics
Lipocalin 1
Chemical analysis
Neurodegenerative diseases
chemical concentration
proteins
body fluids
Body fluids
neurodegenerative diseases
Body Fluids
Muramidase
global change
Electrophoresis
lysozyme

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Changes in the chemical barrier composition of tears in Alzheimer's disease reveal potential tear diagnostic biomarkers. / Kalló, Gergo; Emri, M.; Varga, Zsófia; Ujhelyi, Bernadett; Tőzsér, J.; Csutak, Adrienne; Csosz, Éva.

In: PLoS One, Vol. 11, No. 6, e0158000, 01.06.2016.

Research output: Contribution to journalArticle

Kalló, Gergo ; Emri, M. ; Varga, Zsófia ; Ujhelyi, Bernadett ; Tőzsér, J. ; Csutak, Adrienne ; Csosz, Éva. / Changes in the chemical barrier composition of tears in Alzheimer's disease reveal potential tear diagnostic biomarkers. In: PLoS One. 2016 ; Vol. 11, No. 6.
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