Changes in the Ca2+-transport processes of red cells during cold storage in ACD

I. Szasz, B. Sarkadi, G. Gardos

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Abstract

In order to characterize Ca2+-transport in red cells stored in ACD Ca2+- loading and Mg2+-depletion by the ionophore A23187, CaATPase activity determination in intact cells and an accurate Ca2+-influx technique were adapted to preserved blood. Active Ca2+-efflux (pump) was measured in rejuvenated cells loaded by Ca2+ with A23187. The rate of Ca2+-pump declined only slightly during 3 weeks of storage (from 80±15 to 66±17 μmoles Ca2+/l. cells/min) and a marginal trend of decrease in the Ca:ATP ratio was observed (from 1.96±0.15 to 1.88±0.11). Passive Ca2+-influx (leak) was studied in regenerated cells in which the Ca2+-pump was blocked with 0.2 mM lanthanum. Ca2+-influx showed a slow increase during the first 3 weeks of storage (from 0.4±0.16 to 1.25±0.4 μmoles Ca2+/l. cells/min), later it increased rapidly. Passive Ca2+ leak and exchange transport were studied in unregenerated, phosphate ester-depleted cells. In this case passive Ca2+-influx increased 2-3-fold as early as 1-2 d after storage in ACD. This initial increase was followed by a continuous slow enhancement that reached a flux of 3.5±0.7 μmoles Ca2+/l. cells/min after 3 weeks of storage. The passive Ca2+-permeability increase that occurred during storage could be readily compensated by the Ca2+-pump without causing metabolic imbalance. The Ca2+-transport, of unregenerated stored cells, however, showed impairment under certain conditions (A23187+EDTA and lanthanum treatments, ghost preparation). The Ca2+-induced shape changes were reversible and ran parallel with the cell Ca2+ level during Ca2+-pumping up to 5 weeks of storage. This finding indicates a direct relationship between cell Ca2+ and shape.

Original languageEnglish
Pages (from-to)559-568
Number of pages10
JournalBritish Journal of Haematology
Volume39
Issue number4
Publication statusPublished - 1978

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Calcimycin
Lanthanum
Cell Shape
Ionophores
Edetic Acid
Permeability
Esters
Adenosine Triphosphate
Phosphates

ASJC Scopus subject areas

  • Hematology

Cite this

Changes in the Ca2+-transport processes of red cells during cold storage in ACD. / Szasz, I.; Sarkadi, B.; Gardos, G.

In: British Journal of Haematology, Vol. 39, No. 4, 1978, p. 559-568.

Research output: Contribution to journalArticle

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