Changes in the Archaea microbial community when the biogas fermenters are fed with protein-rich substrates

Norbert Ács, Etelka Kovács, Roland Wirth, Zoltán Bagi, Orsolya Strang, Zsófia Herbel, Gábor Rákhely, Kornél L. Kovács

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Terminal restriction fragment length polymorphism (T-RFLP) was applied to study the changes in the composition of the methanogens of biogas-producing microbial communities on adaptation to protein-rich monosubstrates such as casein and blood. Specially developed laboratory scale (5-L) continuously stirred tank reactors have been developed and used in these experiments. Sequencing of the appropriate T-RF fragments selected from a methanogen-specific (mcrA gene-based) library revealed that the methanogens responded to the unconventional substrates by changing the community structure. T-RFLP of the 16S rDNA gene confirmed the findings.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalBioresource Technology
Volume131
DOIs
Publication statusPublished - Mar 2013

Keywords

  • 16S rRNA gene
  • Biogas
  • McrA gene
  • Methanogen
  • Protein

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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