Changes in septo-hippocampal projections after lateral entorhinal or combined entorhinal-raphé lesions as studied by anterograde tracing methods

C. Nyakas, P. G M Luiten, B. Balkan, D. G. Spencer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Septal and entorhinal projections to the hippocampus show a considerable overlap in their target structures in the molecular layer of the dentate gyrus (DG) and stratum lacunosum-moleculare of the cornu ammonis (CA). Employing anterograde tracing methods, it was investigated in which way the morphological pattern of the septohippocampal projections were influenced by lateral entorhinal cortex (LEA) lesions. Anterograde filling neurons of from soma to axonal terminals with Phaseolus vulgaris leucoagglutinin (PHA-L) revealed lesion-induced changes in innervation patterns in the DG but not in CA fields. LEA lesions provoke an impressive shift of septo-dentate projections from a predominant middle molecular layer innervation to the outer molecular layer, whereas septal projections to the CA remain unchanged. Comparison with concurrent acetylcholinesterase (AChE) staining and immunocytochemical demonstration of choline acetyltransferase (ChAT) confirm the cholinergic nature of this plasticity response. This response was equally strong in unilateral or bilateral damage to the LEA and was neither enhanced nor inhibited by simultaneous injury to the median raphé nuclei.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalBrain Research Bulletin
Volume21
Issue number2
DOIs
Publication statusPublished - 1988

Fingerprint

Entorhinal Cortex
Hippocampus
Dentate Gyrus
Choline O-Acetyltransferase
Carisoprodol
Acetylcholinesterase
Molecular Structure
Cholinergic Agents
Staining and Labeling
Neurons
Wounds and Injuries

Keywords

  • Anterograde tracing
  • Cholinergic
  • Plasticity
  • Septo-hippocampal projections

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Changes in septo-hippocampal projections after lateral entorhinal or combined entorhinal-raphé lesions as studied by anterograde tracing methods. / Nyakas, C.; Luiten, P. G M; Balkan, B.; Spencer, D. G.

In: Brain Research Bulletin, Vol. 21, No. 2, 1988, p. 285-293.

Research output: Contribution to journalArticle

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