Changes in rat muscle with compensatory overload occur in a sequential manner

P. C D Macpherson, R. E. Thayer, Carol Rodgers, A. Taylor, Earl G. Noble

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study was initiated to determine the time course of changes in the profile of selected skeletal muscle myofibril proteins during compensatory overload. Whole muscle isometric contractile properties were measured to assess the physiological consequences of the overload stimulus. Compensatory overload of plantaris muscle of rats was induced by surgical ablation of the synergistic soleus and gastrocnemius muscles. Myosin light chain (LC) and tropomyosin (TM) compositions of control (CP) and overloaded plantaris (OP) muscles were determined by electrophoresis and myofibrillar ATPase assays were performed to assess changes in contractile protein interactions. Within one week of overload decreases in the α:β TM ratio and myofibrillar ATPase activity were observed. Following 30 days of overload, a transition in type II to type I fibres was associated with an increase in slow myosin LC1. Interestingly, after 77 days of overload, the TM subunit ratio returned to one resembling a fast twitch muscle. It is proposed that the early and transitory changes in the TM subunits of OP, as well as the rapid initial depression in maximum tetanic isometric force and myofibrillar ATPase activity may be explained as a result of muscle fibre degeneration-regeneration. We propose that alterations in protein expression induced by compensatory overload reflect both degenerative-regenerative change and increased neuromuscular activity.

Original languageEnglish
Pages (from-to)111-125
Number of pages15
JournalActa Physiologica Hungarica
Volume86
Issue number2
Publication statusPublished - 1999

Fingerprint

Tropomyosin
Skeletal Muscle
Muscles
Adenosine Triphosphatases
Contractile Proteins
Myosin Light Chains
Muscle Proteins
Myofibrils
Myosins
Electrophoresis
Regeneration
Proteins

Keywords

  • Muscle contraction
  • Muscle damage
  • Myofibrillar ATPase
  • Myosin
  • Tropomyosin

ASJC Scopus subject areas

  • Physiology

Cite this

Macpherson, P. C. D., Thayer, R. E., Rodgers, C., Taylor, A., & Noble, E. G. (1999). Changes in rat muscle with compensatory overload occur in a sequential manner. Acta Physiologica Hungarica, 86(2), 111-125.

Changes in rat muscle with compensatory overload occur in a sequential manner. / Macpherson, P. C D; Thayer, R. E.; Rodgers, Carol; Taylor, A.; Noble, Earl G.

In: Acta Physiologica Hungarica, Vol. 86, No. 2, 1999, p. 111-125.

Research output: Contribution to journalArticle

Macpherson, PCD, Thayer, RE, Rodgers, C, Taylor, A & Noble, EG 1999, 'Changes in rat muscle with compensatory overload occur in a sequential manner', Acta Physiologica Hungarica, vol. 86, no. 2, pp. 111-125.
Macpherson, P. C D ; Thayer, R. E. ; Rodgers, Carol ; Taylor, A. ; Noble, Earl G. / Changes in rat muscle with compensatory overload occur in a sequential manner. In: Acta Physiologica Hungarica. 1999 ; Vol. 86, No. 2. pp. 111-125.
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