Changes in PACAP immunoreactivity in human milk and presence of PAC1 receptor in mammary gland during lactation

Katalin Csanaky, Eszter Banki, K. Szabadfi, D. Reglodi, Ibolya Tarcai, Levente Czegledi, Z. Helyes, Tibor Ertl, Judit Gyarmati, Zalan Szanto, Istvan Zapf, Erika Sipos, Seiji Shioda, A. Tamás

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence in the nervous system and peripheral organs, including the mammary gland. Previously, we have shown that PACAP38 is present in the human milk at higher levels than in respective blood samples. However, it is not known how PACAP levels and the expression of PAC1 receptor change during lactation. Therefore, the aim of our study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in human colostrums and transitional and mature milk during lactation and to compare the expression of PAC1 receptors in lactating and non-lactating mammary glands. We found that PACAP38-LI was significantly higher in human colostrum samples than in the transitional and mature milk. PACAP38- LI did not show any significant changes within the first 10-month period of lactation, but a significant increase was observed thereafter, up to the examined 17th month. Weak expression of PAC1 receptors was detected in nonlactating sheep and human mammary glands, but a significant increase was observed in the lactating sheep samples. In summary, the present study is the first to show changes of PACAP levels in human milk during lactation. The presence of PACAP in the milk suggests a potential role in the development of newborn, while the increased expressions of PAC1 receptors on lactating breast may indicate a PACAP38/PAC1 interaction in the mammary gland during lactation.

Original languageEnglish
Pages (from-to)631-637
Number of pages7
JournalJournal of Molecular Neuroscience
Volume48
Issue number3
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Pituitary Adenylate Cyclase-Activating Polypeptide
Human Milk
Human Mammary Glands
Lactation
Milk
Sheep
Colostrum
Peripheral Nervous System
Neuropeptides
Breast

Keywords

  • Human milk
  • Mammary gland
  • PAC1
  • PACAP38

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Changes in PACAP immunoreactivity in human milk and presence of PAC1 receptor in mammary gland during lactation. / Csanaky, Katalin; Banki, Eszter; Szabadfi, K.; Reglodi, D.; Tarcai, Ibolya; Czegledi, Levente; Helyes, Z.; Ertl, Tibor; Gyarmati, Judit; Szanto, Zalan; Zapf, Istvan; Sipos, Erika; Shioda, Seiji; Tamás, A.

In: Journal of Molecular Neuroscience, Vol. 48, No. 3, 11.2012, p. 631-637.

Research output: Contribution to journalArticle

Csanaky, Katalin ; Banki, Eszter ; Szabadfi, K. ; Reglodi, D. ; Tarcai, Ibolya ; Czegledi, Levente ; Helyes, Z. ; Ertl, Tibor ; Gyarmati, Judit ; Szanto, Zalan ; Zapf, Istvan ; Sipos, Erika ; Shioda, Seiji ; Tamás, A. / Changes in PACAP immunoreactivity in human milk and presence of PAC1 receptor in mammary gland during lactation. In: Journal of Molecular Neuroscience. 2012 ; Vol. 48, No. 3. pp. 631-637.
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AU - Banki, Eszter

AU - Szabadfi, K.

AU - Reglodi, D.

AU - Tarcai, Ibolya

AU - Czegledi, Levente

AU - Helyes, Z.

AU - Ertl, Tibor

AU - Gyarmati, Judit

AU - Szanto, Zalan

AU - Zapf, Istvan

AU - Sipos, Erika

AU - Shioda, Seiji

AU - Tamás, A.

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AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence in the nervous system and peripheral organs, including the mammary gland. Previously, we have shown that PACAP38 is present in the human milk at higher levels than in respective blood samples. However, it is not known how PACAP levels and the expression of PAC1 receptor change during lactation. Therefore, the aim of our study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in human colostrums and transitional and mature milk during lactation and to compare the expression of PAC1 receptors in lactating and non-lactating mammary glands. We found that PACAP38-LI was significantly higher in human colostrum samples than in the transitional and mature milk. PACAP38- LI did not show any significant changes within the first 10-month period of lactation, but a significant increase was observed thereafter, up to the examined 17th month. Weak expression of PAC1 receptors was detected in nonlactating sheep and human mammary glands, but a significant increase was observed in the lactating sheep samples. In summary, the present study is the first to show changes of PACAP levels in human milk during lactation. The presence of PACAP in the milk suggests a potential role in the development of newborn, while the increased expressions of PAC1 receptors on lactating breast may indicate a PACAP38/PAC1 interaction in the mammary gland during lactation.

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