Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children

Zsuzsanna Ökrös, E. Endreffy, Zoltan Novak, Zoltan Maroti, Peter Monostori, I. Varga, Agnes Király, S. Túri

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aim: Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment. Main methods: Oxidative stress parameters via photometric methods, CYBB and HMOX-1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children. Key findings: Higher blood thiobarbituric acid reactive substances (TBARS) (p <0.03) and carbonylated protein (p <0.05) levels were found in the asthmatic children than in the controls. The relative expression of CYBB was significantly lower (p <0.05) in patients treated with a low daily dose of inhaled corticosteroid (ICS), than in asthmatics not receiving ICS therapy. Higher ICS doses alone or combined with long acting β2-receptor agonists did not influence the expression significantly. No similar tendency was found as regards to HMOX-1 expression. Significance: Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.

Original languageEnglish
Pages (from-to)907-911
Number of pages5
JournalLife Sciences
Volume91
Issue number19-20
DOIs
Publication statusPublished - Nov 2 2012

Fingerprint

Oxidative stress
NADPH Oxidase
Heme Oxygenase-1
Adrenal Cortex Hormones
Oxidative Stress
Blood
Messenger RNA
Thiobarbituric Acid Reactive Substances
Asthma
Antioxidants
Biomolecules
Gene expression
Isoenzymes
Free Radicals
Real-Time Polymerase Chain Reaction
Proteins
Leukocytes
Therapeutics
Inflammation
Lipids

Keywords

  • Blood
  • Bronchial asthma
  • Inhaled corticosteroids
  • NADPH oxidase

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children. / Ökrös, Zsuzsanna; Endreffy, E.; Novak, Zoltan; Maroti, Zoltan; Monostori, Peter; Varga, I.; Király, Agnes; Túri, S.

In: Life Sciences, Vol. 91, No. 19-20, 02.11.2012, p. 907-911.

Research output: Contribution to journalArticle

Ökrös, Zsuzsanna ; Endreffy, E. ; Novak, Zoltan ; Maroti, Zoltan ; Monostori, Peter ; Varga, I. ; Király, Agnes ; Túri, S. / Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children. In: Life Sciences. 2012 ; Vol. 91, No. 19-20. pp. 907-911.
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AU - Maroti, Zoltan

AU - Monostori, Peter

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AB - Aim: Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment. Main methods: Oxidative stress parameters via photometric methods, CYBB and HMOX-1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children. Key findings: Higher blood thiobarbituric acid reactive substances (TBARS) (p <0.03) and carbonylated protein (p <0.05) levels were found in the asthmatic children than in the controls. The relative expression of CYBB was significantly lower (p <0.05) in patients treated with a low daily dose of inhaled corticosteroid (ICS), than in asthmatics not receiving ICS therapy. Higher ICS doses alone or combined with long acting β2-receptor agonists did not influence the expression significantly. No similar tendency was found as regards to HMOX-1 expression. Significance: Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.

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