Changes in microcirculation after ischemic process in rat skeletal muscle

N. Németh, Tamas Lesznyak, E. Bráth, Gabor Acs, Arpad Nagy, Jozsef Pap-Szekeres, I. Furka, I. Mikó

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Laser Doppler flowmetry (LDF) is a good method to investigate tissue microcirculation, but it has many standardization and measuring problems. To exclude these effects, we performed a test using LDF on rat skeletal muscle. In 12 CD outbred anesthetized rats, bilateral femoral vessels and the quadriceps femoris muscle were exposed. The left femoral artery and vein were clamped for 1 h by microvascular clips (ischemic side). On the right side, no other intervention was made (control side). An LDF probe was applied on the medial vastus muscle. Short-term occlusions (2-3 s) were performed before and after the 1-h clamping and on the control side while LDF curves were registered. The halftimes of ascending curves on the ischemic side were significantly elongated vs. the condition before clamping (P = 0.0007) or the control side (P = 0.0017). Microcirculatory changes caused by 1-h ischemia were shown by this simple, quick, and well-reproducible test on rat skeletal muscle.

Original languageEnglish
Pages (from-to)419-423
Number of pages5
JournalMicrosurgery
Volume23
Issue number5
DOIs
Publication statusPublished - 2003

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Laser-Doppler Flowmetry
Microcirculation
Skeletal Muscle
Quadriceps Muscle
Constriction
Femoral Vein
Femoral Artery
Thigh
Surgical Instruments
Ischemia
Muscles

ASJC Scopus subject areas

  • Surgery

Cite this

Changes in microcirculation after ischemic process in rat skeletal muscle. / Németh, N.; Lesznyak, Tamas; Bráth, E.; Acs, Gabor; Nagy, Arpad; Pap-Szekeres, Jozsef; Furka, I.; Mikó, I.

In: Microsurgery, Vol. 23, No. 5, 2003, p. 419-423.

Research output: Contribution to journalArticle

Németh, N. ; Lesznyak, Tamas ; Bráth, E. ; Acs, Gabor ; Nagy, Arpad ; Pap-Szekeres, Jozsef ; Furka, I. ; Mikó, I. / Changes in microcirculation after ischemic process in rat skeletal muscle. In: Microsurgery. 2003 ; Vol. 23, No. 5. pp. 419-423.
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