Changes in binding of monoclonal antibodies to TSH receptor following thyrotropin (TSH) and gonadotropin (GTH) pretreatments (studies on human thyroid and CHO cell lines)

S. U. Nagy, G. Csaba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Monoclonal antibody to TSH-receptor binds to the cells of human thyroid gland and to Chinese hamster ovary cell cultures. Pretreatments with TSH can decrease the binding of antibody, which effect is more expressed in CHO than in thyroid cells. Gonadotropin (FSH + LH) cannot influence the binding of antibody to thyroid, but there is an effect on CHO cells. Endotoxin (lipopolysaccharide, LPS) can depress the binding of antibodies on both objects and abolishes the difference between thyrotropin and gonadotropin receptors in their binding capacity, on both cell types. The experiments prove the similarity of thyrotropin and gonadotropin receptors which is responsible for the hormonal overlap.

Original languageEnglish
Pages (from-to)339-348
Number of pages10
JournalActa Physiologica Hungarica
Volume82
Issue number4
Publication statusPublished - 1994

Fingerprint

Thyrotropin Receptors
CHO Cells
Thyrotropin
Gonadotropin Receptors
Gonadotropins
Thyroid Gland
Monoclonal Antibodies
Cell Line
Antibodies
Cricetulus
Endotoxins
Lipopolysaccharides
Ovary
Cell Culture Techniques

Keywords

  • CHO cell line
  • hormonal imprinting
  • receptor-antibody
  • thyroid gland
  • TSH-gonadotropin overlap
  • TSH-receptor

ASJC Scopus subject areas

  • Physiology

Cite this

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AU - Csaba, G.

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AB - Monoclonal antibody to TSH-receptor binds to the cells of human thyroid gland and to Chinese hamster ovary cell cultures. Pretreatments with TSH can decrease the binding of antibody, which effect is more expressed in CHO than in thyroid cells. Gonadotropin (FSH + LH) cannot influence the binding of antibody to thyroid, but there is an effect on CHO cells. Endotoxin (lipopolysaccharide, LPS) can depress the binding of antibodies on both objects and abolishes the difference between thyrotropin and gonadotropin receptors in their binding capacity, on both cell types. The experiments prove the similarity of thyrotropin and gonadotropin receptors which is responsible for the hormonal overlap.

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