Changes in apoplast protein pattern suggest an early role of cell wall structure remodelling in flagellin-triggered basal immunity

E. Szabó, A. Szatmári, E. Hunyadi-Gulyás, E. Besenyei, L. R. Zsiros, Z. Bozsó, P. G. Ott

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The leaf apoplast is a dynamic compartment in contact with plant pathogenic bacteria after infection. Among the very first interaction events is the receptor-mediated perception of bacterial surface molecules such as flagellin or other conserved microbe-associated molecular patterns (MAMPs). Apoplast proteins likely play a role in basal resistance (BR) or pattern-triggered immunity (PTI). Here, a proteomic approach was carried out on water soluble - potentially the most mobile - apoplast proteins from flagellin-treated tobacco (Nicotiana tabacum) leaves. As the quickness of BR/PTI seems crucial for its efficacy, samples were taken as early as 2. 5 and 7 h post inoculation. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Forty-nine different proteins from 28 protein spots changed in their density compared to the water-inoculated control. Eleven protein spots appeared de novo in response to EBR induction. There are glycohydrolases and redox-active proteins besides pathogenesis-related proteins among them, predicting plant cell wall structural modifications and more direct antimicrobial effectors as earliest changes related to BR/PTI.

Original languageEnglish
Pages (from-to)551-559
Number of pages9
JournalBiologia Plantarum
Volume56
Issue number3
DOIs
Publication statusPublished - Sep 1 2012

Keywords

  • basal resistance
  • glycohydrolases
  • pathogenesis-related proteins
  • proteomics
  • tobacco

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

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