Changes in alkylation damage removal during in vitro neuronal differentiation

A. Czibula, Gyöngyi Leiker, I. Raskó

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mouse teratocarcinoma cell lines allow the analysis of very early commitment and differentiation events, that are likely to be similar to those operating in the early mouse embryo. We have previously characterised the excision repair capabilities of these cells after ultraviolet light irradiation and found that differentiation is accompanied by reduction of excision repair. In the present study we examine the operation of an other DNA repair pathway participating in the removal of alkylation damage. O6-alkylguanine-DNA alkyltransferase (ATase) activity was determined in undifferentiated and differentiated mouse P19 teratocarcinoma cell line. To obtain more information about regulation of ATase we transfected P19 cells with constructs harbouring a human ATase cDNA driven by a housekeeping promoter.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalActa Biologica Hungarica
Volume48
Issue number1
Publication statusPublished - 1997

Fingerprint

alkylation
Alkylation
Alkyl and Aryl Transferases
DNA Repair
repair
Teratocarcinoma
Repair
damage
mice
Cells
cell lines
DNA
Cell Line
Housekeeping
Ultraviolet Rays
DNA repair
ultraviolet radiation
embryo
embryo (animal)
irradiation

Keywords

  • DNA repair
  • In vitro neuronal differentiation
  • O-alkylguanine-DNA alkyltransferase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Changes in alkylation damage removal during in vitro neuronal differentiation. / Czibula, A.; Leiker, Gyöngyi; Raskó, I.

In: Acta Biologica Hungarica, Vol. 48, No. 1, 1997, p. 113-120.

Research output: Contribution to journalArticle

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