Following insulin pretreatment (hormonal imprinting) of Tetrahymena cells there was an increased binding of insulin both in the plasma membrane and in the nuclear envelope of the offspring generations. A short (1 x 15 min) treatment did not produce imprinting in the plasma membrane but a 1 h treatment was sufficient. A similar short (1 x 15 min) treatment was enough to develop imprinting in the nuclear envelope and this is the treatment which induced the highest response. In the nuclear membrane there was a significant difference in the effect of 4 x 15 min and 1 x 60 min treatments but there was no effect in the plasma membrane. The effect of imprinting was observed after 48 h (16-20 generations). The repeated treatment of imprinted cells did not induce a further increase in the binding of the hormone.
|Number of pages||6|
|Publication status||Published - 1994|
ASJC Scopus subject areas
- Aquatic Science
- Cell Biology