Challenging tumour immunological techniques that help to track cancer stem cells in malignant melanomas and other solid tumours

Beatrix Kotlan, Vanda Plotar, Klara Eles, Szabolcs Horvath, Timea Balatoni, Orsolya Csuka, Mihaly Újhelyi, Ákos Sávolt, Andras Szollar, Istvan Vamosi-Nagy, Laszlo Toth, Emil Farkas, Jozsef Toth, Miklos Kasler, Gabriella Liszkay

Research output: Contribution to journalArticle

Abstract

Aim of the study: The arsenal of questions and answers about the minor cancer initiating cancer stem cell (CSC) population put responsible for cancer invasiveness and metastases, has left with an unsolved puzzle. Specific aims of a complex project were partly focused on revealing new biomarkers of cancer. We designed and set up novel techniques to facilitate the detection of cancerous cells. Materials and methods: As a novel approach, we investigated B cells infiltrating breast carcinomas and melanomas (TIL-B) in terms of their tumour antigen binding potential. By developing the TIL-B phage display technology we provide here a new technology for the specific detection of highly tumour-associated antigens. Single chain Fv (scFv) antibody fragment phage ELISA, immunofluorescence (IF) FACS analysis, chamber slide technique with IF confocal laser microscopy and immunohistochemistry (IHC) in paraffin-embedded tissue sections were set up and standardized. Results: We showed strong tumour-associated disialylated glycosphingolipid expression levels on various cancer cells using scFv antibody fragments, generated previously by uniquely invasive breast carcinoma TIL-B phage display library technology. Conclusions: We report herein a novel strategy to obtain antibody fragments of human origin that recognise tumour-associated ganglioside antigens. Our investigations have the power to detect privileged molecules in cancer progression, invasiveness, and metastases. The technical achievements of this study are being harnessed for early diagnostics and effective cancer therapeutics.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalWspolczesna Onkologia
Volume2
Issue number1A
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Cancer stem cells
  • Malignant melanomas
  • Technical developments
  • Tumour immunology

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging

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    Kotlan, B., Plotar, V., Eles, K., Horvath, S., Balatoni, T., Csuka, O., Újhelyi, M., Sávolt, Á., Szollar, A., Vamosi-Nagy, I., Toth, L., Farkas, E., Toth, J., Kasler, M., & Liszkay, G. (2017). Challenging tumour immunological techniques that help to track cancer stem cells in malignant melanomas and other solid tumours. Wspolczesna Onkologia, 2(1A), 41-47. https://doi.org/10.5114/wo.2018.73884