Cervus elaphus papillomavirus (CePV1): New insights on viral evolution in deer

A. Scagliarini, L. Gallina, M. Battilani, F. Turrini, F. Savini, A. Lavazza, M. Chiari, E. Coradduzza, A. Peli, K. Erdélyi, A. Alberti

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We identified a novel papillomavirus (CePV1) in a fibropapilloma of a 1.5 year old male red deer (Cervus elaphus) shot in the Italian Alps in Brescia province. PV particles were first observed by electron microscopy and PV DNA was then identified by PCR using degenerate primers. Subsequently we cloned the entire genome and determined its complete sequence. CePV1 genome is 8009. bp long and contains all 9 ORFs and the long untranslated regulatory region characteristic for Delta-papillomaviruses. Pairwise nucleotide alignments and phylogenetic analyses based on concatenated E1-E2-L1 ORFs allowed to determine the highest similarity with the Capreolus caprelus papillomavirus CcaPV1. The analysis of the host-parasite phylogenetic tree interactions suggest the co-divergence of CePV1 and C. elaphus while the identified topological incongruences leading us to speculate that CcaPV1 could eventually be the result of an earlier host switch event.

Original languageEnglish
Pages (from-to)252-259
Number of pages8
JournalVeterinary Microbiology
Volume165
Issue number3-4
DOIs
Publication statusPublished - Aug 30 2013

Fingerprint

Deer
Papillomaviridae
Cervus elaphus
deer
Open Reading Frames
Genome
Untranslated Regions
Deltapapillomavirus
open reading frames
Nucleic Acid Regulatory Sequences
Capreolus
Electron Microscopy
Parasites
genome
Nucleotides
phylogeny
Polymerase Chain Reaction
DNA
electron microscopy
nucleotides

Keywords

  • Cervus elaphus
  • Evolution
  • Genome
  • Papillomavirus
  • Red deer

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Scagliarini, A., Gallina, L., Battilani, M., Turrini, F., Savini, F., Lavazza, A., ... Alberti, A. (2013). Cervus elaphus papillomavirus (CePV1): New insights on viral evolution in deer. Veterinary Microbiology, 165(3-4), 252-259. https://doi.org/10.1016/j.vetmic.2013.03.012

Cervus elaphus papillomavirus (CePV1) : New insights on viral evolution in deer. / Scagliarini, A.; Gallina, L.; Battilani, M.; Turrini, F.; Savini, F.; Lavazza, A.; Chiari, M.; Coradduzza, E.; Peli, A.; Erdélyi, K.; Alberti, A.

In: Veterinary Microbiology, Vol. 165, No. 3-4, 30.08.2013, p. 252-259.

Research output: Contribution to journalArticle

Scagliarini, A, Gallina, L, Battilani, M, Turrini, F, Savini, F, Lavazza, A, Chiari, M, Coradduzza, E, Peli, A, Erdélyi, K & Alberti, A 2013, 'Cervus elaphus papillomavirus (CePV1): New insights on viral evolution in deer', Veterinary Microbiology, vol. 165, no. 3-4, pp. 252-259. https://doi.org/10.1016/j.vetmic.2013.03.012
Scagliarini A, Gallina L, Battilani M, Turrini F, Savini F, Lavazza A et al. Cervus elaphus papillomavirus (CePV1): New insights on viral evolution in deer. Veterinary Microbiology. 2013 Aug 30;165(3-4):252-259. https://doi.org/10.1016/j.vetmic.2013.03.012
Scagliarini, A. ; Gallina, L. ; Battilani, M. ; Turrini, F. ; Savini, F. ; Lavazza, A. ; Chiari, M. ; Coradduzza, E. ; Peli, A. ; Erdélyi, K. ; Alberti, A. / Cervus elaphus papillomavirus (CePV1) : New insights on viral evolution in deer. In: Veterinary Microbiology. 2013 ; Vol. 165, No. 3-4. pp. 252-259.
@article{674a5d6fd3364637b767eb3df026656b,
title = "Cervus elaphus papillomavirus (CePV1): New insights on viral evolution in deer",
abstract = "We identified a novel papillomavirus (CePV1) in a fibropapilloma of a 1.5 year old male red deer (Cervus elaphus) shot in the Italian Alps in Brescia province. PV particles were first observed by electron microscopy and PV DNA was then identified by PCR using degenerate primers. Subsequently we cloned the entire genome and determined its complete sequence. CePV1 genome is 8009. bp long and contains all 9 ORFs and the long untranslated regulatory region characteristic for Delta-papillomaviruses. Pairwise nucleotide alignments and phylogenetic analyses based on concatenated E1-E2-L1 ORFs allowed to determine the highest similarity with the Capreolus caprelus papillomavirus CcaPV1. The analysis of the host-parasite phylogenetic tree interactions suggest the co-divergence of CePV1 and C. elaphus while the identified topological incongruences leading us to speculate that CcaPV1 could eventually be the result of an earlier host switch event.",
keywords = "Cervus elaphus, Evolution, Genome, Papillomavirus, Red deer",
author = "A. Scagliarini and L. Gallina and M. Battilani and F. Turrini and F. Savini and A. Lavazza and M. Chiari and E. Coradduzza and A. Peli and K. Erd{\'e}lyi and A. Alberti",
year = "2013",
month = "8",
day = "30",
doi = "10.1016/j.vetmic.2013.03.012",
language = "English",
volume = "165",
pages = "252--259",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Cervus elaphus papillomavirus (CePV1)

T2 - New insights on viral evolution in deer

AU - Scagliarini, A.

AU - Gallina, L.

AU - Battilani, M.

AU - Turrini, F.

AU - Savini, F.

AU - Lavazza, A.

AU - Chiari, M.

AU - Coradduzza, E.

AU - Peli, A.

AU - Erdélyi, K.

AU - Alberti, A.

PY - 2013/8/30

Y1 - 2013/8/30

N2 - We identified a novel papillomavirus (CePV1) in a fibropapilloma of a 1.5 year old male red deer (Cervus elaphus) shot in the Italian Alps in Brescia province. PV particles were first observed by electron microscopy and PV DNA was then identified by PCR using degenerate primers. Subsequently we cloned the entire genome and determined its complete sequence. CePV1 genome is 8009. bp long and contains all 9 ORFs and the long untranslated regulatory region characteristic for Delta-papillomaviruses. Pairwise nucleotide alignments and phylogenetic analyses based on concatenated E1-E2-L1 ORFs allowed to determine the highest similarity with the Capreolus caprelus papillomavirus CcaPV1. The analysis of the host-parasite phylogenetic tree interactions suggest the co-divergence of CePV1 and C. elaphus while the identified topological incongruences leading us to speculate that CcaPV1 could eventually be the result of an earlier host switch event.

AB - We identified a novel papillomavirus (CePV1) in a fibropapilloma of a 1.5 year old male red deer (Cervus elaphus) shot in the Italian Alps in Brescia province. PV particles were first observed by electron microscopy and PV DNA was then identified by PCR using degenerate primers. Subsequently we cloned the entire genome and determined its complete sequence. CePV1 genome is 8009. bp long and contains all 9 ORFs and the long untranslated regulatory region characteristic for Delta-papillomaviruses. Pairwise nucleotide alignments and phylogenetic analyses based on concatenated E1-E2-L1 ORFs allowed to determine the highest similarity with the Capreolus caprelus papillomavirus CcaPV1. The analysis of the host-parasite phylogenetic tree interactions suggest the co-divergence of CePV1 and C. elaphus while the identified topological incongruences leading us to speculate that CcaPV1 could eventually be the result of an earlier host switch event.

KW - Cervus elaphus

KW - Evolution

KW - Genome

KW - Papillomavirus

KW - Red deer

UR - http://www.scopus.com/inward/record.url?scp=84892952136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892952136&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2013.03.012

DO - 10.1016/j.vetmic.2013.03.012

M3 - Article

C2 - 23578708

AN - SCOPUS:84892952136

VL - 165

SP - 252

EP - 259

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -