Cerulenin-induced Changes in Lipid and Fatty Acid Content of Chloroplasts in Detached Greening Barley Leaves

G. Laskay, T. Farkas, E. Lehoczki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Measurements were made of the monogalactosyl diglyceride, digalactosyl diglyceride, total phospholipid, and total fatty acid contents, the fatty acid compositions of the glycerolipids of plastids from detached barley leaves before and after 72 hours of greening in the presence or absence of the fatty acid biosynthesis inhibitor cerulenin. At the final stage of greening the incorporation of 1-14C-acetate into leaf tissue and that of U-14C-galactose into chloroplast galactolipids as well as the ultrastructure of chloroplasts were also studied. Cerulenin caused a marked reduction in the rate of accumulation of chloroplast lipids and fatty acids during the greening period. In addition, specific alterations were observed in the fatty acid composition of monogalactosyl diglyceride, phosphatidyl choline, and phosphatidyl inositol following cerulenin treatment, the possible reasons of which are discussed. 1-14C-acetate incorporation into leaf slices was only slightly reduced in cerulenin-treated leaves after 72 hours of greening, whereas nearly 3 times as much U-14C-galactose incorporated into chloroplast galactolipids as in the control. The chloroplasts of cerulenin-treated leaves displayed a marked membrane deficiency, probably due to the shortage of galactolipids.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalJournal of Plant Physiology
Volume118
Issue number3
DOIs
Publication statusPublished - Jan 1 1985

Fingerprint

Cerulenin
Hordeum
Chloroplasts
Galactolipids
galactolipids
Fatty Acids
chloroplasts
fatty acid composition
barley
Diglycerides
Lipids
diacylglycerols
lipids
leaves
Galactose
galactose
Acetates
acetates
fatty acids
Plastids

Keywords

  • 16:0
  • 18:2
  • 18:3
  • 3-/3,4-dichlorophenyV-1,1-dimethylurea
  • ACP
  • acyl carrier protein
  • biosynthesis of galactolipids
  • cerulenin
  • chloroplast lamellar structure
  • chloroplast lipids
  • DCMU
  • DEGS
  • DGDG
  • diethyleneglycol-succinate
  • digalactosyl diglyceride
  • fatty acid composition
  • Hordeum vulgare
  • linoleic acid
  • linolenic acid
  • MGDG
  • monogalactosyl diglyceride
  • palmitic acid
  • PC
  • PG
  • phosphatidyl choline
  • phosphatidyl glycerol
  • phosphatidyl inositol
  • PI
  • SL
  • sulfolipid

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Cerulenin-induced Changes in Lipid and Fatty Acid Content of Chloroplasts in Detached Greening Barley Leaves. / Laskay, G.; Farkas, T.; Lehoczki, E.

In: Journal of Plant Physiology, Vol. 118, No. 3, 01.01.1985, p. 267-275.

Research output: Contribution to journalArticle

@article{2f57fbce95c3467a945045e971862844,
title = "Cerulenin-induced Changes in Lipid and Fatty Acid Content of Chloroplasts in Detached Greening Barley Leaves",
abstract = "Measurements were made of the monogalactosyl diglyceride, digalactosyl diglyceride, total phospholipid, and total fatty acid contents, the fatty acid compositions of the glycerolipids of plastids from detached barley leaves before and after 72 hours of greening in the presence or absence of the fatty acid biosynthesis inhibitor cerulenin. At the final stage of greening the incorporation of 1-14C-acetate into leaf tissue and that of U-14C-galactose into chloroplast galactolipids as well as the ultrastructure of chloroplasts were also studied. Cerulenin caused a marked reduction in the rate of accumulation of chloroplast lipids and fatty acids during the greening period. In addition, specific alterations were observed in the fatty acid composition of monogalactosyl diglyceride, phosphatidyl choline, and phosphatidyl inositol following cerulenin treatment, the possible reasons of which are discussed. 1-14C-acetate incorporation into leaf slices was only slightly reduced in cerulenin-treated leaves after 72 hours of greening, whereas nearly 3 times as much U-14C-galactose incorporated into chloroplast galactolipids as in the control. The chloroplasts of cerulenin-treated leaves displayed a marked membrane deficiency, probably due to the shortage of galactolipids.",
keywords = "16:0, 18:2, 18:3, 3-/3,4-dichlorophenyV-1,1-dimethylurea, ACP, acyl carrier protein, biosynthesis of galactolipids, cerulenin, chloroplast lamellar structure, chloroplast lipids, DCMU, DEGS, DGDG, diethyleneglycol-succinate, digalactosyl diglyceride, fatty acid composition, Hordeum vulgare, linoleic acid, linolenic acid, MGDG, monogalactosyl diglyceride, palmitic acid, PC, PG, phosphatidyl choline, phosphatidyl glycerol, phosphatidyl inositol, PI, SL, sulfolipid",
author = "G. Laskay and T. Farkas and E. Lehoczki",
year = "1985",
month = "1",
day = "1",
doi = "10.1016/S0176-1617(85)80228-5",
language = "English",
volume = "118",
pages = "267--275",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "3",

}

TY - JOUR

T1 - Cerulenin-induced Changes in Lipid and Fatty Acid Content of Chloroplasts in Detached Greening Barley Leaves

AU - Laskay, G.

AU - Farkas, T.

AU - Lehoczki, E.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Measurements were made of the monogalactosyl diglyceride, digalactosyl diglyceride, total phospholipid, and total fatty acid contents, the fatty acid compositions of the glycerolipids of plastids from detached barley leaves before and after 72 hours of greening in the presence or absence of the fatty acid biosynthesis inhibitor cerulenin. At the final stage of greening the incorporation of 1-14C-acetate into leaf tissue and that of U-14C-galactose into chloroplast galactolipids as well as the ultrastructure of chloroplasts were also studied. Cerulenin caused a marked reduction in the rate of accumulation of chloroplast lipids and fatty acids during the greening period. In addition, specific alterations were observed in the fatty acid composition of monogalactosyl diglyceride, phosphatidyl choline, and phosphatidyl inositol following cerulenin treatment, the possible reasons of which are discussed. 1-14C-acetate incorporation into leaf slices was only slightly reduced in cerulenin-treated leaves after 72 hours of greening, whereas nearly 3 times as much U-14C-galactose incorporated into chloroplast galactolipids as in the control. The chloroplasts of cerulenin-treated leaves displayed a marked membrane deficiency, probably due to the shortage of galactolipids.

AB - Measurements were made of the monogalactosyl diglyceride, digalactosyl diglyceride, total phospholipid, and total fatty acid contents, the fatty acid compositions of the glycerolipids of plastids from detached barley leaves before and after 72 hours of greening in the presence or absence of the fatty acid biosynthesis inhibitor cerulenin. At the final stage of greening the incorporation of 1-14C-acetate into leaf tissue and that of U-14C-galactose into chloroplast galactolipids as well as the ultrastructure of chloroplasts were also studied. Cerulenin caused a marked reduction in the rate of accumulation of chloroplast lipids and fatty acids during the greening period. In addition, specific alterations were observed in the fatty acid composition of monogalactosyl diglyceride, phosphatidyl choline, and phosphatidyl inositol following cerulenin treatment, the possible reasons of which are discussed. 1-14C-acetate incorporation into leaf slices was only slightly reduced in cerulenin-treated leaves after 72 hours of greening, whereas nearly 3 times as much U-14C-galactose incorporated into chloroplast galactolipids as in the control. The chloroplasts of cerulenin-treated leaves displayed a marked membrane deficiency, probably due to the shortage of galactolipids.

KW - 16:0

KW - 18:2

KW - 18:3

KW - 3-/3,4-dichlorophenyV-1,1-dimethylurea

KW - ACP

KW - acyl carrier protein

KW - biosynthesis of galactolipids

KW - cerulenin

KW - chloroplast lamellar structure

KW - chloroplast lipids

KW - DCMU

KW - DEGS

KW - DGDG

KW - diethyleneglycol-succinate

KW - digalactosyl diglyceride

KW - fatty acid composition

KW - Hordeum vulgare

KW - linoleic acid

KW - linolenic acid

KW - MGDG

KW - monogalactosyl diglyceride

KW - palmitic acid

KW - PC

KW - PG

KW - phosphatidyl choline

KW - phosphatidyl glycerol

KW - phosphatidyl inositol

KW - PI

KW - SL

KW - sulfolipid

UR - http://www.scopus.com/inward/record.url?scp=0043150625&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0043150625&partnerID=8YFLogxK

U2 - 10.1016/S0176-1617(85)80228-5

DO - 10.1016/S0176-1617(85)80228-5

M3 - Article

VL - 118

SP - 267

EP - 275

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 3

ER -