Cerium-mediated free radicals in contractile proteins. EPR and DSC study

M. Kiss, F. Könczöl, N. Farkas, D. Lőrinczy, J. Belágyi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of free radicals obtained in hydroxyl and cerium(IV)-nitrilotriacetic acid free radical generating systems on contractile proteins (actin, myosin and their complexes in glycerinated muscle fibres) was studied using differential scanning calorimetry and spin trapping electron paramagnetic resonance technique. The analysis of spectra showed that selective attack of thiol groups - Cys-257 and Cys-374 residues of actin, and among others Cys-707 residue of myosin - and random attack of sidechains of the main proteins of muscle tissue produced structural and functional changes, which affected the ATP hydrolysis cycle and very likely the dynamics of actin. The melting curves obtained on protein systems support the view that global conformational changes accompany the local damage of free radicals.

Original languageEnglish
Pages (from-to)627-637
Number of pages11
JournalJournal of Thermal Analysis and Calorimetry
Volume65
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Cerium
Contractile Proteins
cerium
Free radicals
free radicals
Free Radicals
Paramagnetic resonance
myosins
Actins
Myosins
proteins
attack
Muscle
Nitrilotriacetic Acid
muscle fibers
Proteins
support systems
Muscle Proteins
adenosine triphosphate
Adenosinetriphosphate

Keywords

  • Contractile proteins
  • DSC
  • Hydroxyl and thiyl free radicals
  • Spin-trapping EPR

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Cerium-mediated free radicals in contractile proteins. EPR and DSC study. / Kiss, M.; Könczöl, F.; Farkas, N.; Lőrinczy, D.; Belágyi, J.

In: Journal of Thermal Analysis and Calorimetry, Vol. 65, No. 2, 2001, p. 627-637.

Research output: Contribution to journalArticle

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