Cerebrovascular pathology in hypertriglyceridemic APOB-100 transgenic mice

Z. Hoyk, Melinda E. Tóth, Nikolett Lénárt, Dóra Nagy, Brigitta Dukay, Alexandra Csefová, A. Zvara, György Seprényi, András Kincses, Fruzsina R. Walter, S. Veszelka, Judit Vígh, Beáta Barabási, András Harazin, A. Kittel, L. Puskás, B. Penke, L. Vígh, Mária A. Deli, M. Sántha

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hypertriglyceridemia is not only a serious risk factor in the development of cardiovascular diseases, but it is linked to neurodegeneration, too. Previously, we generated transgenic mice overexpressing the human APOB-100 protein, a mouse model of human atherosclerosis. In this model we observed high plasma levels of triglycerides, oxidative stress, tau hyperphosphorylation, synaptic dysfunction, cognitive impairment, increased neural apoptosis and neurodegeneration. Neurovascular dysfunction is recognized as a key factor in the development of neurodegenerative diseases, but the cellular and molecular events linking cerebrovascular pathology and neurodegeneration are not fully understood. Our aim was to study cerebrovascular changes in APOB-100 transgenic mice. We described the kinetics of the development of chronic hypertriglyceridemia in the transgenic animals. Increased blood-brain barrier permeability was found in the hippocampus of APOB-100 transgenic mice which was accompanied by structural changes. Using transmission electron microscopy, we detected changes in the brain capillary endothelial tight junction structure and edematous swelling of astrocyte endfeet. In brain microvessels isolated from APOB-100 transgenic animals increased Lox-1, Aqp4, and decreased Meox-2, Mfsd2a, Abcb1a, Lrp2, Glut-1, Nos2, Nos3, Vim, and in transgenic brains reduced Cdh2 and Gfap-σ gene expressions were measured using quantitative real-time PCR. We confirmed the decreased P-glycoprotein (ABCB1) and vimentin expression related to the neurovascular unit by immunostaining in transgenic brain sections using confocal microscopy. We conclude that in chronic hypertriglyceridemic APOB-100 transgenic mice both functional and morphological cerebrovascular pathology can be observed, and this animal model could be a useful tool to study the link between cerebrovascular pathology and neurodegeneration.

Original languageEnglish
Article number380
JournalFrontiers in Cellular Neuroscience
Volume12
DOIs
Publication statusPublished - Oct 25 2018

Fingerprint

Transgenic Mice
Pathology
Genetically Modified Animals
Hypertriglyceridemia
Brain
Tight Junctions
P-Glycoprotein
Vimentin
Microvessels
Blood-Brain Barrier
Transmission Electron Microscopy
Confocal Microscopy
Astrocytes
Neurodegenerative Diseases
Real-Time Polymerase Chain Reaction
Permeability
Hippocampus
Atherosclerosis
Triglycerides
Oxidative Stress

Keywords

  • Apolipoprotein B-100
  • Astroglia
  • Blood-brain barrier
  • Brain endothelial cell
  • Cerebrovascular pathology
  • Hypertriglyceridemia
  • P-glycoprotein
  • Tight junction

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Cerebrovascular pathology in hypertriglyceridemic APOB-100 transgenic mice. / Hoyk, Z.; Tóth, Melinda E.; Lénárt, Nikolett; Nagy, Dóra; Dukay, Brigitta; Csefová, Alexandra; Zvara, A.; Seprényi, György; Kincses, András; Walter, Fruzsina R.; Veszelka, S.; Vígh, Judit; Barabási, Beáta; Harazin, András; Kittel, A.; Puskás, L.; Penke, B.; Vígh, L.; Deli, Mária A.; Sántha, M.

In: Frontiers in Cellular Neuroscience, Vol. 12, 380, 25.10.2018.

Research output: Contribution to journalArticle

Hoyk, Z, Tóth, ME, Lénárt, N, Nagy, D, Dukay, B, Csefová, A, Zvara, A, Seprényi, G, Kincses, A, Walter, FR, Veszelka, S, Vígh, J, Barabási, B, Harazin, A, Kittel, A, Puskás, L, Penke, B, Vígh, L, Deli, MA & Sántha, M 2018, 'Cerebrovascular pathology in hypertriglyceridemic APOB-100 transgenic mice', Frontiers in Cellular Neuroscience, vol. 12, 380. https://doi.org/10.3389/fncel.2018.00380
Hoyk, Z. ; Tóth, Melinda E. ; Lénárt, Nikolett ; Nagy, Dóra ; Dukay, Brigitta ; Csefová, Alexandra ; Zvara, A. ; Seprényi, György ; Kincses, András ; Walter, Fruzsina R. ; Veszelka, S. ; Vígh, Judit ; Barabási, Beáta ; Harazin, András ; Kittel, A. ; Puskás, L. ; Penke, B. ; Vígh, L. ; Deli, Mária A. ; Sántha, M. / Cerebrovascular pathology in hypertriglyceridemic APOB-100 transgenic mice. In: Frontiers in Cellular Neuroscience. 2018 ; Vol. 12.
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KW - Tight junction

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