Cerebral Microbleeds Temporarily Become Less Visible or Invisible in Acute Susceptibility Weighted Magnetic Resonance Imaging: A Rat Study

Arnold Tóth, Z. Berente, P. Bogner, Bálint Környei, Bendegúz Balogh, Endre Czeiter, Krisztina Amrein, T. Dóczi, A. Büki, Attila Schwarcz

Research output: Contribution to journalArticle

Abstract

Previously, we reported human traumatic brain injury cases demonstrating acute to subacute microbleed appearance changes in susceptibility-weighted imaging (SWI - magnetic resonance imaging [MRI]). This study aims to confirm and characterize such temporal microbleed appearance alterations in an experimental model. To elicit microbleed formation, brains of male Sprague Dawley rats were pierced in a depth of 4 mm, in a parasagittal position bilaterally using 159 μm and 474 μm needles, without the injection of autologous blood or any agent. Rats underwent 4.7 T MRI immediately, then at multiple time points until 125 h. Volumes of hypointensities consistent with microbleeds in SWI were measured using an intensity threshold-based approach. Microbleed volumes across time points were compared using repeated measures analysis of variance. Microbleeds were assessed by Prussian blue histology at different time points. Hypointensity volumes referring to microbleeds were significantly decreased (corrected p < 0.05) at 24 h compared with the immediate or the 125 h time points. By visual inspection, microbleeds were similarly detectable at the immediate and 125 h imaging but were decreased in extent or completely absent at 24 h or 48 h. Histology confirmed the presence of microbleeds at all time points and in all animals. This study confirmed a general temporary reduction in visibility of microbleeds in the acute phase in SWI. Such short-term appearance dynamics of microbleeds should be considered when using SWI as a diagnostic tool for microbleeds in traumatic brain injury and various diseases.

Original languageEnglish
Pages (from-to)1670-1677
Number of pages8
JournalJournal of Neurotrauma
Volume36
Issue number10
DOIs
Publication statusPublished - May 15 2019

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Magnetic Resonance Imaging
Histology
Needles
Sprague Dawley Rats
Analysis of Variance
Theoretical Models
Injections
Brain
Traumatic Brain Injury

Keywords

  • brain trauma
  • microbleed
  • MRI
  • rat
  • susceptibility weighted imaging (SWI)

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Cerebral Microbleeds Temporarily Become Less Visible or Invisible in Acute Susceptibility Weighted Magnetic Resonance Imaging : A Rat Study. / Tóth, Arnold; Berente, Z.; Bogner, P.; Környei, Bálint; Balogh, Bendegúz; Czeiter, Endre; Amrein, Krisztina; Dóczi, T.; Büki, A.; Schwarcz, Attila.

In: Journal of Neurotrauma, Vol. 36, No. 10, 15.05.2019, p. 1670-1677.

Research output: Contribution to journalArticle

Tóth, Arnold ; Berente, Z. ; Bogner, P. ; Környei, Bálint ; Balogh, Bendegúz ; Czeiter, Endre ; Amrein, Krisztina ; Dóczi, T. ; Büki, A. ; Schwarcz, Attila. / Cerebral Microbleeds Temporarily Become Less Visible or Invisible in Acute Susceptibility Weighted Magnetic Resonance Imaging : A Rat Study. In: Journal of Neurotrauma. 2019 ; Vol. 36, No. 10. pp. 1670-1677.
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