Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond

Anna Rusznyák, E. Tóth, Peter Schumann, Cathrin Spröer, Judit Makk, Gitta Szabó, Péter Vladár, K. Márialigeti, A. Borsodi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An alkalitolerant and moderately halophilic strain, designated KB23T, characterized by optimal growth at pH 8.0-9.0 and in the presence of 5-7% (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23 T were Gram-stain-positive, motile straight rods. Strain KB23 T was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (L-Orn-D-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C 15: 0, C 16: 0 and anteiso-C 15: 1 were the major cellular fatty acids. The DNA G+C content of strain KB23 T was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109 T (97.35% similarity), Cellulomonas terrae DB5 T (96.81%), Cellulomonas iranensis O T (96.75), Cellulomonas chitinilytica X.bu-b T (96.60%), Cellulomonas persica IT (96.53%), Cellulomonas composti TR7-06T (96.45%), Cellulomonas biazotea DSM 20112T (96.34%) and Cellulomonas fimi DSM 20113 T (96.20%). According to these results, together with DNA-DNA hybridization and physiological data, strain KB23 T is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23 T (5DSM 22512T 5NCAIM B002303 T).

Original languageEnglish
Pages (from-to)1662-1666
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number7
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Cellulomonas
periphyton
Phragmites australis
pond
Bacteria
DNA
bacterium
new species
bacteria
isoprenoid
Cellulomonas iranensis
Cellulomonas persica
Cellulomonas biazotea
fatty acid
Cellulomonas fimi
Cellulomonas flavigena
phylogenetics
gene
peptidoglycans
isoprenoids

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond. / Rusznyák, Anna; Tóth, E.; Schumann, Peter; Spröer, Cathrin; Makk, Judit; Szabó, Gitta; Vladár, Péter; Márialigeti, K.; Borsodi, A.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 7, 07.2011, p. 1662-1666.

Research output: Contribution to journalArticle

@article{100ae87adad94ae0862377ff9c560440,
title = "Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond",
abstract = "An alkalitolerant and moderately halophilic strain, designated KB23T, characterized by optimal growth at pH 8.0-9.0 and in the presence of 5-7{\%} (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23 T were Gram-stain-positive, motile straight rods. Strain KB23 T was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (L-Orn-D-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C 15: 0, C 16: 0 and anteiso-C 15: 1 were the major cellular fatty acids. The DNA G+C content of strain KB23 T was 74.8 mol{\%}. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109 T (97.35{\%} similarity), Cellulomonas terrae DB5 T (96.81{\%}), Cellulomonas iranensis O T (96.75), Cellulomonas chitinilytica X.bu-b T (96.60{\%}), Cellulomonas persica IT (96.53{\%}), Cellulomonas composti TR7-06T (96.45{\%}), Cellulomonas biazotea DSM 20112T (96.34{\%}) and Cellulomonas fimi DSM 20113 T (96.20{\%}). According to these results, together with DNA-DNA hybridization and physiological data, strain KB23 T is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23 T (5DSM 22512T 5NCAIM B002303 T).",
author = "Anna Ruszny{\'a}k and E. T{\'o}th and Peter Schumann and Cathrin Spr{\"o}er and Judit Makk and Gitta Szab{\'o} and P{\'e}ter Vlad{\'a}r and K. M{\'a}rialigeti and A. Borsodi",
year = "2011",
month = "7",
doi = "10.1099/ijs.0.022608-0",
language = "English",
volume = "61",
pages = "1662--1666",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "7",

}

TY - JOUR

T1 - Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond

AU - Rusznyák, Anna

AU - Tóth, E.

AU - Schumann, Peter

AU - Spröer, Cathrin

AU - Makk, Judit

AU - Szabó, Gitta

AU - Vladár, Péter

AU - Márialigeti, K.

AU - Borsodi, A.

PY - 2011/7

Y1 - 2011/7

N2 - An alkalitolerant and moderately halophilic strain, designated KB23T, characterized by optimal growth at pH 8.0-9.0 and in the presence of 5-7% (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23 T were Gram-stain-positive, motile straight rods. Strain KB23 T was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (L-Orn-D-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C 15: 0, C 16: 0 and anteiso-C 15: 1 were the major cellular fatty acids. The DNA G+C content of strain KB23 T was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109 T (97.35% similarity), Cellulomonas terrae DB5 T (96.81%), Cellulomonas iranensis O T (96.75), Cellulomonas chitinilytica X.bu-b T (96.60%), Cellulomonas persica IT (96.53%), Cellulomonas composti TR7-06T (96.45%), Cellulomonas biazotea DSM 20112T (96.34%) and Cellulomonas fimi DSM 20113 T (96.20%). According to these results, together with DNA-DNA hybridization and physiological data, strain KB23 T is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23 T (5DSM 22512T 5NCAIM B002303 T).

AB - An alkalitolerant and moderately halophilic strain, designated KB23T, characterized by optimal growth at pH 8.0-9.0 and in the presence of 5-7% (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23 T were Gram-stain-positive, motile straight rods. Strain KB23 T was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (L-Orn-D-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C 15: 0, C 16: 0 and anteiso-C 15: 1 were the major cellular fatty acids. The DNA G+C content of strain KB23 T was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109 T (97.35% similarity), Cellulomonas terrae DB5 T (96.81%), Cellulomonas iranensis O T (96.75), Cellulomonas chitinilytica X.bu-b T (96.60%), Cellulomonas persica IT (96.53%), Cellulomonas composti TR7-06T (96.45%), Cellulomonas biazotea DSM 20112T (96.34%) and Cellulomonas fimi DSM 20113 T (96.20%). According to these results, together with DNA-DNA hybridization and physiological data, strain KB23 T is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23 T (5DSM 22512T 5NCAIM B002303 T).

UR - http://www.scopus.com/inward/record.url?scp=79960172846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960172846&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.022608-0

DO - 10.1099/ijs.0.022608-0

M3 - Article

C2 - 20729313

AN - SCOPUS:79960172846

VL - 61

SP - 1662

EP - 1666

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 7

ER -