Cellulase production by T. reesei

K. Réczey, Zs. Szengyel, R. Eklund, G. Zacchi

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were peformed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter. However the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter. The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the feimenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalBioresource Technology
Volume57
Issue number1
DOIs
Publication statusPublished - Jul 1996

Fingerprint

Salix
Cellulase
Steam
endo-1,4-beta-glucanase
steam
cellulose
Cellulose
Fermenters
fermenters
filter
Cellulases
cellulases
fermentation
Fermentation
substrate
Pressure
Trichoderma reesei
batch fermentation
Trichoderma
Lignin

Keywords

  • Cellulases
  • Enzyme production
  • Lignocellulose
  • Steam pretreatment
  • Trichoderma reesei

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Cellulase production by T. reesei. / Réczey, K.; Szengyel, Zs.; Eklund, R.; Zacchi, G.

In: Bioresource Technology, Vol. 57, No. 1, 07.1996, p. 25-30.

Research output: Contribution to journalArticle

Réczey, K. ; Szengyel, Zs. ; Eklund, R. ; Zacchi, G. / Cellulase production by T. reesei. In: Bioresource Technology. 1996 ; Vol. 57, No. 1. pp. 25-30.
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