Cellulase fermentation on a novel substrate (waste cardboard) and subsequent utilization of home-produced cellulase and commercial amylase in a rabbit feeding trial

Nóra Szijártó, Zsófia Faigl, K. Réczey, M. Mézes, András Bersényi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cellulase and amylase enzymes are increasingly being used as feed additives in animal nutrition. Those are mainly applied in order to improve the nutritional value of feed by partially degrading its fiber content, which has major importance during the early post-weaning period of young animals possessing inadequate digestive enzyme activities. Due to their commercial availability at low cost, the utilization of amylases as animal feed additive can be highly recommended, while the high price of cellulases actually makes their use as a feed additive unreasonable. A promising way to gain cheap cellulases is to conduct the enzyme fermentation process on novel substrates, which are readily available at no cost. In this study, the utilization of old corrugated cardboard (OCC) as a novel fermentation substrate has been investigated for cellulase enzyme fermentation by Trichoderma reesei Rut C-30. Batch fermentation experiments were carried out in shake-flasks and in a 30 l laboratory fermenter. The OCC feedstock proved to be a suitable fermentation substrate, a filter paper activity (FPA) of as high as 2.27 FPU ml-1 was achieved in the fermenter, which corresponds to a yield of 227 FPU g -1 cellulose. The maximum productivity achieved was 35.7 FPU l -1 h-1. The subsequent utilization of this cellulase enzyme preparation was investigated as additive of commercial rabbit compound feed in order to improve its digestibility during the early post-weaning period. In parallel, commercial amylase preparations were also tested for the same purposes. The results of animal feeding experiments showed, that enzyme supplementation of the complete feed improved production traits, as it increased the digestibility of nutrients and thus reduced the mortality caused by digestive disorders.

Original languageEnglish
Pages (from-to)49-57
Number of pages9
JournalIndustrial Crops and Products
Volume20
Issue number1
DOIs
Publication statusPublished - Jul 2004

Fingerprint

cardboard
cellulases
Cellulase
Amylases
endo-1,4-beta-glucanase
amylases
Fermentation
feed additives
rabbits
fermentation
Rabbits
fermenters
Enzymes
Cellulases
weaning
enzymes
digestibility
Weaning
Trichoderma reesei
batch fermentation

Keywords

  • Amylase
  • Cellulase
  • Enzyme production
  • Rabbit diet
  • Trichoderma reesei

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science

Cite this

Cellulase fermentation on a novel substrate (waste cardboard) and subsequent utilization of home-produced cellulase and commercial amylase in a rabbit feeding trial. / Szijártó, Nóra; Faigl, Zsófia; Réczey, K.; Mézes, M.; Bersényi, András.

In: Industrial Crops and Products, Vol. 20, No. 1, 07.2004, p. 49-57.

Research output: Contribution to journalArticle

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