Cellulase enzyme production by various fungal strains on different carbon sources

M. Bollók, K. Réczey

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the process of making ethanol from lignocellulosics, enzyme production is still the most crucial and costly step. Trichoderma reesei Rut C-30 is the best known cellulase producer. This fungus is very good in endo-and exoglucanase production, however, the amount of β-glucosidase excreted is not sufficient for hydrolysis. In addition, T. reesei Rut C-30 is sensitive to inhibitors generated during steam-pretreatment of wood. In the present study, two good cellulase producers were selected from 16 fungal strains and investigated regarding filter paper activity (FPU) and β-glucosidase activity using inexpensive lignocellulosic carbon sources for cultivation. T. reesei Rut C-30 proved to be a good cellulase producer, resulting in a maximum FPU of 1.39 FPU ml-1 on washed, steam-pretreated willow, but the β-glucosidase activity was insufficient. High β-glucosidase activities were reached with T. viride OKI B1 on all substrates, with a maximum activity of 1.62 IU ml-1 on steam-pretreated spruce.

Original languageEnglish
Pages (from-to)155-168
Number of pages14
JournalActa Alimentaria
Volume29
Issue number2
Publication statusPublished - Jun 2000

Fingerprint

Glucosidases
glucosidases
cellulases
Cellulase
Trichoderma reesei
Steam
Carbon
endo-1,4-beta-glucanase
steam
carbon
Enzymes
Salix
Trichoderma
Picea
Hydrolysis
Fungi
Ethanol
pretreatment
hydrolysis
ethanol

Keywords

  • Cellulase production
  • Paper sludge
  • Steam pretreated wood
  • Trichoderma

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Cellulase enzyme production by various fungal strains on different carbon sources. / Bollók, M.; Réczey, K.

In: Acta Alimentaria, Vol. 29, No. 2, 06.2000, p. 155-168.

Research output: Contribution to journalArticle

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