Cellular localisation of the proteins of region 3 of feline enteric coronavirus

István Mészáros, Ferenc Olasz, Enikő Kádár-Hürkecz, Ádám Bálint, Ákos Hornyák, Sándor Belák, Zoltán Zádori

Research output: Contribution to journalArticle

Abstract

Feline enteric coronaviruses have three open reading frames (ORFs) in region 3 (3a, 3b, and 3c). All three ORFs were expressed with C-terminal eGFP and 3xFLAG tags in different cell lines and their localisation was determined. ORF 3a is predicted to contain DNA-binding and transcription activator domains, and it is localised in the nucleus and in the cytoplasm. ORF 3b is also predicted to contain DNA-binding and activator domains, and was found to localise in the mitochondrion. Besides that, in some of the non-infected and FIPV-infected cells nucleolar, perinuclear or nuclear membrane accumulation of the eGFP-tagged 3b was observed. The exact compartmental localisation of ORF 3c is yet to be determined. However, based on our co-localisation studies 3c does not seem to be localised in the ER-Golgi network, ERGIC or peroxisomes. The expression of 3c-eGFP is clearly cell type dependent, it is more stable in MARC 145 cells than in Fcwf-4 or CrFK cells, which might reflect in vivo stability differences of 3c in natural target cells (enterocytes vs. monocytes/macrophages).

Original languageEnglish
Pages (from-to)493-508
Number of pages16
JournalActa veterinaria Hungarica
Volume66
Issue number3
DOIs
Publication statusPublished - Sep 2018

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Keywords

  • Coronavirus
  • FECV
  • FIPV
  • Mitochondrial localisation
  • Nucleolar localisation
  • ORF

ASJC Scopus subject areas

  • veterinary(all)

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