Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens

P. Balogh, Zsuzsa Bebök, P. Németh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalJournal of Immunological Methods
Volume153
Issue number1-2
DOIs
Publication statusPublished - Aug 30 1992

Fingerprint

Hybridomas
Surface Antigens
Antibodies
Enzymes
Immobilized Cells
Glutaral
Thymocytes
Bone Marrow Cells
Thymus Gland
Lysine
Monoclonal Antibodies
Cell Line

Keywords

  • Circle assay
  • ELISA, cellular
  • Hybridoma screening
  • Immunocytochemistry

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens. / Balogh, P.; Bebök, Zsuzsa; Németh, P.

In: Journal of Immunological Methods, Vol. 153, No. 1-2, 30.08.1992, p. 141-149.

Research output: Contribution to journalArticle

@article{60d7ecc74c12476a820a5304095b0332,
title = "Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens",
abstract = "A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.",
keywords = "Circle assay, ELISA, cellular, Hybridoma screening, Immunocytochemistry",
author = "P. Balogh and Zsuzsa Beb{\"o}k and P. N{\'e}meth",
year = "1992",
month = "8",
day = "30",
doi = "10.1016/0022-1759(92)90316-L",
language = "English",
volume = "153",
pages = "141--149",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens

AU - Balogh, P.

AU - Bebök, Zsuzsa

AU - Németh, P.

PY - 1992/8/30

Y1 - 1992/8/30

N2 - A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.

AB - A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.

KW - Circle assay

KW - ELISA, cellular

KW - Hybridoma screening

KW - Immunocytochemistry

UR - http://www.scopus.com/inward/record.url?scp=0026774396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026774396&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(92)90316-L

DO - 10.1016/0022-1759(92)90316-L

M3 - Article

C2 - 1517584

AN - SCOPUS:0026774396

VL - 153

SP - 141

EP - 149

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -