Abstract
A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.
| Original language | English |
|---|---|
| Pages (from-to) | 141-149 |
| Number of pages | 9 |
| Journal | Journal of Immunological Methods |
| Volume | 153 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Aug 30 1992 |
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Keywords
- Circle assay
- ELISA, cellular
- Hybridoma screening
- Immunocytochemistry
ASJC Scopus subject areas
- Biotechnology
- Immunology
Cite this
Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens. / Balogh, P.; Bebök, Zsuzsa; Németh, P.
In: Journal of Immunological Methods, Vol. 153, No. 1-2, 30.08.1992, p. 141-149.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular enzyme-linked immunocircle assay. A rapid assay of hybridomas produced against cell surface antigens
AU - Balogh, P.
AU - Bebök, Zsuzsa
AU - Németh, P.
PY - 1992/8/30
Y1 - 1992/8/30
N2 - A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.
AB - A novel method has been developed for the initial screening of hybridomas produced against cell surface antigens. Glutaraldehyde-fixed cells were immobilized as targets on the lid of a 96-well tissue culture plate which had been precoated with poly-l-lysine. Antibody binding was determined by an immunoenzymatic method in an arrangement permitting both macro- and microscopic examination. After optimization with mouse thymus cells using existing rat monoclonal antibodies, new rat-mouse hybridoma cell lines against mouse thymocytes and bone marrow cells were screened. The antibodies could be characterized immediately both by the localization of the immune reaction (surface or intracellular) or as estimated by the frequency of positive cells recognized by the antibody in the sample.
KW - Circle assay
KW - ELISA, cellular
KW - Hybridoma screening
KW - Immunocytochemistry
UR - http://www.scopus.com/inward/record.url?scp=0026774396&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026774396&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(92)90316-L
DO - 10.1016/0022-1759(92)90316-L
M3 - Article
C2 - 1517584
AN - SCOPUS:0026774396
VL - 153
SP - 141
EP - 149
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -