Cellular electrophysiological effects of platelet-activating factor (PAF) and its antagonist BN 52021 in cardiac preparations

V. Kecskemeti, P. Braquet

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8 Citations (Scopus)

Abstract

The effects of PAF and its antagonist BN 52021 were studied on the transmembrane action potential (AP) in atrial and ventricular papillary muscles of guinea-pig. PAF (10-11-10-7 M) did not modify the resting membrane potential (RP) nor the maximum rate of depolarization (V(max)) either in atrial or in ventricular fibres. At 10 -11 M, PAF increased the amplitude of AP both in atrial and ventricular muscles. The repolarization phase was dose-dependently shortened in the Case of atrium, while the duration of ventricular AP was somewhat increased. The K+ channel blocker 4-aminopyridine (10-3 M) prevented the effect of PAF on the duration of atrial AP. BN 52021 (10-7M to 10-5 M) produced a significant shortening of the duration of atrial AP and did not modify the other parameters. In papillary muscle up to 10-6M, it increased both RP and the amplitude of AP and caused a dose-dependent shortening of the repolarization. Neither PAF (10 -11 to 10-7M) nor BN 52021 (10-5 M) was able to induce slow AP in guinea-pig atrial and ventricular preparations depolarized by 25mM K+ Tyrode solution. PAF did not modify the slow AP elicited by isoprenaline (5 x 10 -7 M). The present findings suggest that neither PAF nor BN 52021 affects slow inward Ca2+ current but their effects on other ionic currents, e.g. K+ currents, may be important.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalDrugs under Experimental and Clinical Research
Volume18
Issue number1
Publication statusPublished - 1992

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ginkgolide B
Platelet Activating Factor
Action Potentials
Membrane Potentials
Papillary Muscles
Guinea Pigs
4-Aminopyridine
Isoproterenol

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology (medical)
  • Drug Discovery
  • Pharmacology

Cite this

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abstract = "The effects of PAF and its antagonist BN 52021 were studied on the transmembrane action potential (AP) in atrial and ventricular papillary muscles of guinea-pig. PAF (10-11-10-7 M) did not modify the resting membrane potential (RP) nor the maximum rate of depolarization (V(max)) either in atrial or in ventricular fibres. At 10 -11 M, PAF increased the amplitude of AP both in atrial and ventricular muscles. The repolarization phase was dose-dependently shortened in the Case of atrium, while the duration of ventricular AP was somewhat increased. The K+ channel blocker 4-aminopyridine (10-3 M) prevented the effect of PAF on the duration of atrial AP. BN 52021 (10-7M to 10-5 M) produced a significant shortening of the duration of atrial AP and did not modify the other parameters. In papillary muscle up to 10-6M, it increased both RP and the amplitude of AP and caused a dose-dependent shortening of the repolarization. Neither PAF (10 -11 to 10-7M) nor BN 52021 (10-5 M) was able to induce slow AP in guinea-pig atrial and ventricular preparations depolarized by 25mM K+ Tyrode solution. PAF did not modify the slow AP elicited by isoprenaline (5 x 10 -7 M). The present findings suggest that neither PAF nor BN 52021 affects slow inward Ca2+ current but their effects on other ionic currents, e.g. K+ currents, may be important.",
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N2 - The effects of PAF and its antagonist BN 52021 were studied on the transmembrane action potential (AP) in atrial and ventricular papillary muscles of guinea-pig. PAF (10-11-10-7 M) did not modify the resting membrane potential (RP) nor the maximum rate of depolarization (V(max)) either in atrial or in ventricular fibres. At 10 -11 M, PAF increased the amplitude of AP both in atrial and ventricular muscles. The repolarization phase was dose-dependently shortened in the Case of atrium, while the duration of ventricular AP was somewhat increased. The K+ channel blocker 4-aminopyridine (10-3 M) prevented the effect of PAF on the duration of atrial AP. BN 52021 (10-7M to 10-5 M) produced a significant shortening of the duration of atrial AP and did not modify the other parameters. In papillary muscle up to 10-6M, it increased both RP and the amplitude of AP and caused a dose-dependent shortening of the repolarization. Neither PAF (10 -11 to 10-7M) nor BN 52021 (10-5 M) was able to induce slow AP in guinea-pig atrial and ventricular preparations depolarized by 25mM K+ Tyrode solution. PAF did not modify the slow AP elicited by isoprenaline (5 x 10 -7 M). The present findings suggest that neither PAF nor BN 52021 affects slow inward Ca2+ current but their effects on other ionic currents, e.g. K+ currents, may be important.

AB - The effects of PAF and its antagonist BN 52021 were studied on the transmembrane action potential (AP) in atrial and ventricular papillary muscles of guinea-pig. PAF (10-11-10-7 M) did not modify the resting membrane potential (RP) nor the maximum rate of depolarization (V(max)) either in atrial or in ventricular fibres. At 10 -11 M, PAF increased the amplitude of AP both in atrial and ventricular muscles. The repolarization phase was dose-dependently shortened in the Case of atrium, while the duration of ventricular AP was somewhat increased. The K+ channel blocker 4-aminopyridine (10-3 M) prevented the effect of PAF on the duration of atrial AP. BN 52021 (10-7M to 10-5 M) produced a significant shortening of the duration of atrial AP and did not modify the other parameters. In papillary muscle up to 10-6M, it increased both RP and the amplitude of AP and caused a dose-dependent shortening of the repolarization. Neither PAF (10 -11 to 10-7M) nor BN 52021 (10-5 M) was able to induce slow AP in guinea-pig atrial and ventricular preparations depolarized by 25mM K+ Tyrode solution. PAF did not modify the slow AP elicited by isoprenaline (5 x 10 -7 M). The present findings suggest that neither PAF nor BN 52021 affects slow inward Ca2+ current but their effects on other ionic currents, e.g. K+ currents, may be important.

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