Cellular and subcellular localization of the mGluR5a metabotropic glutamate receptor in rat spinal cord

ZÓltan Vidnyánszky, Jozsef Hámori, Lászlo Négyessy, Doris RÜegg, Thomas KnÖpfel, Rainer Kuhn, Tamás J. GÖrcs

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The cellular and subcellular distribution of the mGluR5a metabotropic glutamate receptor was studied in the spinal cord of the rat using an antibody raised against a mGluR5a-specific carboxy-terminal peptide. Strong mGluR5a-immunoreactivity (mGluR5a-ir) was found in the laminae I—II of the dorsal horn, which gradually decreased towards the deeper layers. At the electron microscopical level, mGluR5a-ir was present exclusively in neuronal somata and dendrites. Immunometal labelling revealed that mGluR5a-ir is concentrated at the periphery of postsynaptic densities of asymmetrical synapses or localized extrasynaptically at dendritic and somatic membranes. The mGluR5a-immunoreactive dendritic profiles were often targeted by synaptic boutons with the morphological characteristics of C-fibre terminals. These observations provide evidence for mGluR5a being involved in the nociceptive transmission at the dorsal horn.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
Issue number1
Publication statusPublished - Dec 1994



  • Antibody
  • Dorsal horn
  • Immunohistochemistry
  • Nociception
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

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