Cellular and subcellular distribution of spinophilin, a PP1 regulatory protein that bundles F-actin in dendritic spines

Charles C. Ouimet, István Katona, Patrick Allen, Tamás F. Freund, Paul Greengard

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Spinophilin is an actin binding protein that positions protein phosphatase 1 next to its substrates in dendritic spines. It contains a single PDZ domain and has the biochemical characteristics of a cytoskeletal scaffolding protein. Previous studies suggest that spinophilin is present in most spines, but the concentration of spinophilin varies from brain region to region in a manner that does not simply reflect differences in spine density. Here, we show that spinophilin is enriched in the great majority of dendritic spines in cerebral cortex, caudatoputamen, hippocampal formation, and cerebellum, irrespective of regional differences in spinophilin concentration. In addition, spinophilin is present postsynaptic to asymmetrical contacts on interneuronal dendritic shafts. We further show that, in hippocampus and ventral pallidum, spinophilin is occasionally present in dendritic shafts adjacent to γ-aminobutyric acid-containing contacts. Thus, the functional role of spinophilin may not be exclusively restricted to excitatory synapses and may be significant at a small fraction of inhibitory contacts. These data also suggest that the concentration of spinophilin per spine is variable and is likely regulated by local physiological factors and/or regional influences.

Original languageEnglish
Pages (from-to)374-388
Number of pages15
JournalJournal of Comparative Neurology
Volume479
Issue number4
DOIs
Publication statusPublished - Nov 22 2004

Keywords

  • Plasticity
  • Synapse
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

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