Cellular adhesion molecules in rat adjuvant arthritis

Margaret M. Halloran, Z. Szekanecz, Nora Barquin, G. Kenneth Haines, Alisa E. Koch

Research output: Contribution to journalArticle

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Abstract

Objective. To examine adhesion molecule expression during the progression of inflammation in a rheumatoid arthritis model of adjuvant-induced arthritis (AIA) in rats. Methods. Immunohistochemical analysis was used to determine the distribution of the following adhesion molecules: lymphocyte function- associated antigen 1 (LFA-1; CD11a/CD18), Mac-1 and p150/95 (CD11bc/CD18), intercellular adhesion molecule 1 (ICAM-1), and CD44 in tissue sections from the ankle joints of rats with AIA. Control animals and those with AIA were killed at intervals over a 54-day period after injection with mineral oil and Mycobacterium butyricum, respectively. Results. CD44 and LFA-1 were expressed on lymphocytes, macrophages, and synovial (ST) lining cells. CD44 expression on macrophages was found to be increased compared with control animals by day 18, and was significantly increased by day 41. CD44 expression on lymphocytes significantly increased earlier, on days 11-18. Increased LFA-1 expression on macrophages occurred late, on day 41. LFA-1 expression on lymphocytes was significantly increased on days 25, 47, and 54. ST lining cells exhibited two distinct periods of increased expression, one early, on days 11-25 and one later, on days 41-54. CD11b/c was expressed on macrophages and ST lining cells, showing a significant increase on AIA rat ST lining cells compared with control animals on day 4. No differences in ICAM-1 expression on endothelial cells between rats with AIA and controls were found on any of the days examined. Conclusion. CD44 expression is up-regulated on macrophages and lymphocytes during the early development of AIA, while LFA-1 expression is up-regulated later in the development of AIA. The up-regulation of CD44 and LFA-1 at different times in the development of AIA suggests an important role for these adhesion molecules in establishing and sustaining an inflammatory response in the AIA joint.

Original languageEnglish
Pages (from-to)810-819
Number of pages10
JournalArthritis and Rheumatism
Volume39
Issue number5
DOIs
Publication statusPublished - May 1996

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Experimental Arthritis
Lymphocyte Function-Associated Antigen-1
Macrophages
Lymphocytes
Intercellular Adhesion Molecule-1
Mineral Oil
Ankle Joint
Mycobacterium
Rheumatoid Arthritis
Up-Regulation
Endothelial Cells
Joints
Inflammation
Injections

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Halloran, M. M., Szekanecz, Z., Barquin, N., Haines, G. K., & Koch, A. E. (1996). Cellular adhesion molecules in rat adjuvant arthritis. Arthritis and Rheumatism, 39(5), 810-819. https://doi.org/10.1002/art.1780390514

Cellular adhesion molecules in rat adjuvant arthritis. / Halloran, Margaret M.; Szekanecz, Z.; Barquin, Nora; Haines, G. Kenneth; Koch, Alisa E.

In: Arthritis and Rheumatism, Vol. 39, No. 5, 05.1996, p. 810-819.

Research output: Contribution to journalArticle

Halloran, MM, Szekanecz, Z, Barquin, N, Haines, GK & Koch, AE 1996, 'Cellular adhesion molecules in rat adjuvant arthritis', Arthritis and Rheumatism, vol. 39, no. 5, pp. 810-819. https://doi.org/10.1002/art.1780390514
Halloran, Margaret M. ; Szekanecz, Z. ; Barquin, Nora ; Haines, G. Kenneth ; Koch, Alisa E. / Cellular adhesion molecules in rat adjuvant arthritis. In: Arthritis and Rheumatism. 1996 ; Vol. 39, No. 5. pp. 810-819.
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abstract = "Objective. To examine adhesion molecule expression during the progression of inflammation in a rheumatoid arthritis model of adjuvant-induced arthritis (AIA) in rats. Methods. Immunohistochemical analysis was used to determine the distribution of the following adhesion molecules: lymphocyte function- associated antigen 1 (LFA-1; CD11a/CD18), Mac-1 and p150/95 (CD11bc/CD18), intercellular adhesion molecule 1 (ICAM-1), and CD44 in tissue sections from the ankle joints of rats with AIA. Control animals and those with AIA were killed at intervals over a 54-day period after injection with mineral oil and Mycobacterium butyricum, respectively. Results. CD44 and LFA-1 were expressed on lymphocytes, macrophages, and synovial (ST) lining cells. CD44 expression on macrophages was found to be increased compared with control animals by day 18, and was significantly increased by day 41. CD44 expression on lymphocytes significantly increased earlier, on days 11-18. Increased LFA-1 expression on macrophages occurred late, on day 41. LFA-1 expression on lymphocytes was significantly increased on days 25, 47, and 54. ST lining cells exhibited two distinct periods of increased expression, one early, on days 11-25 and one later, on days 41-54. CD11b/c was expressed on macrophages and ST lining cells, showing a significant increase on AIA rat ST lining cells compared with control animals on day 4. No differences in ICAM-1 expression on endothelial cells between rats with AIA and controls were found on any of the days examined. Conclusion. CD44 expression is up-regulated on macrophages and lymphocytes during the early development of AIA, while LFA-1 expression is up-regulated later in the development of AIA. The up-regulation of CD44 and LFA-1 at different times in the development of AIA suggests an important role for these adhesion molecules in establishing and sustaining an inflammatory response in the AIA joint.",
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AB - Objective. To examine adhesion molecule expression during the progression of inflammation in a rheumatoid arthritis model of adjuvant-induced arthritis (AIA) in rats. Methods. Immunohistochemical analysis was used to determine the distribution of the following adhesion molecules: lymphocyte function- associated antigen 1 (LFA-1; CD11a/CD18), Mac-1 and p150/95 (CD11bc/CD18), intercellular adhesion molecule 1 (ICAM-1), and CD44 in tissue sections from the ankle joints of rats with AIA. Control animals and those with AIA were killed at intervals over a 54-day period after injection with mineral oil and Mycobacterium butyricum, respectively. Results. CD44 and LFA-1 were expressed on lymphocytes, macrophages, and synovial (ST) lining cells. CD44 expression on macrophages was found to be increased compared with control animals by day 18, and was significantly increased by day 41. CD44 expression on lymphocytes significantly increased earlier, on days 11-18. Increased LFA-1 expression on macrophages occurred late, on day 41. LFA-1 expression on lymphocytes was significantly increased on days 25, 47, and 54. ST lining cells exhibited two distinct periods of increased expression, one early, on days 11-25 and one later, on days 41-54. CD11b/c was expressed on macrophages and ST lining cells, showing a significant increase on AIA rat ST lining cells compared with control animals on day 4. No differences in ICAM-1 expression on endothelial cells between rats with AIA and controls were found on any of the days examined. Conclusion. CD44 expression is up-regulated on macrophages and lymphocytes during the early development of AIA, while LFA-1 expression is up-regulated later in the development of AIA. The up-regulation of CD44 and LFA-1 at different times in the development of AIA suggests an important role for these adhesion molecules in establishing and sustaining an inflammatory response in the AIA joint.

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