Cells with regulatory function of the innate and adaptive immune system in primary Sjögren's syndrome: ORIGINAL ARTICLE

P. Szodoray, G. Papp, I. F. Horvath, S. Baráth, S. Sipka, B. Nakken, M. Zeher

Research output: Contribution to journalArticle

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Abstract

Summary The aim of the present study was to describe subsets of cells with regulatory properties in primary Sjögren's syndrome (pSS), and to correlate these cell populations with clinical symptoms. Among the 32 investigated patients, 23 had extraglandular manifestations (EGMs), while nine had only glandular symptoms. Twenty healthy individuals served as controls. The percentages of natural killer (NK), natural killer T cells (NK T), interleukin (IL)-10 producing T regulatory type 1 (Tr1) cells and CD4+CD25 + regulatory T cells (Treg) cells were determined by flow cytometry and serum cytokine levels of IL-4, IL-6, IL-10, tumour necrosis factor (TNF)-α and interferon (IFN)-γ were evaluated by enzyme-linked immunosorbent assay (ELISA). Functional tests were carried out to assess the suppressor properties of Treg cells in patients and controls. Peripheral NK, NK T and Tr1 cell percentages were elevated in pSS, while CD4+CD25+ Treg cells showed reduced frequencies in patients compared to controls. In pSS, elevated percentages of NK T, Tr1 and CD4+CD25+ Treg cells were observed in patients with EGMs, when compared to patients with sicca symptoms only. CD4 +CD25+ Treg cell percentages showed a negative correlation with sialometry values. The in vitro functional assay demonstrated lower suppression activity of CD4+CD25+ Treg cells in patients compared to controls. Serum IL-6 and TNF-α levels were elevated, while IL-10 was decreased in patients compared to controls. Negative correlation was found between IL-10 levels and the percentages of Tr1 cells. Changes in the investigated subsets of regulatory cells in pSS may contribute to the development and progression of the disease.

Original languageEnglish
Pages (from-to)343-349
Number of pages7
JournalClinical and Experimental Immunology
Volume157
Issue number3
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Regulatory T-Lymphocytes
Immune System
Interleukin-10
Interleukin-6
Tumor Necrosis Factor-alpha
Natural Killer T-Cells
Serum
Interleukin-4
Interferons
Disease Progression
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Cytokines

Keywords

  • Innate and adaptive immune system
  • Regulatory cells
  • Sjögren's syndrome

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Cells with regulatory function of the innate and adaptive immune system in primary Sjögren's syndrome : ORIGINAL ARTICLE. / Szodoray, P.; Papp, G.; Horvath, I. F.; Baráth, S.; Sipka, S.; Nakken, B.; Zeher, M.

In: Clinical and Experimental Immunology, Vol. 157, No. 3, 09.2009, p. 343-349.

Research output: Contribution to journalArticle

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