Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultrastructure

Anna Geleta, Z. Kristóf, A. Maráz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of Schizosaccahromyces pombe RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.

Original languageEnglish
Pages (from-to)35-46
Number of pages12
JournalActa Microbiologica et Immunologica Hungarica
Volume54
Issue number1
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Flocculation
Schizosaccharomyces
Cell Wall
Enzymes
Digestion
Endopeptidase K
Pronase
Lectins
Cell Communication
Peptide Hydrolases
Ethanol
Maintenance
Electrons
Proteins

Keywords

  • Cell wall
  • Electron-microscopy
  • Flocculation
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)
  • Microbiology

Cite this

@article{df35102d91d345ef8a83898acd18dad2,
title = "Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultrastructure",
abstract = "Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of Schizosaccahromyces pombe RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6{\%} (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.",
keywords = "Cell wall, Electron-microscopy, Flocculation, Schizosaccharomyces pombe",
author = "Anna Geleta and Z. Krist{\'o}f and A. Mar{\'a}z",
year = "2007",
month = "3",
doi = "10.1556/AMicr.54.2007.1.4",
language = "English",
volume = "54",
pages = "35--46",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultrastructure

AU - Geleta, Anna

AU - Kristóf, Z.

AU - Maráz, A.

PY - 2007/3

Y1 - 2007/3

N2 - Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of Schizosaccahromyces pombe RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.

AB - Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of Schizosaccahromyces pombe RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.

KW - Cell wall

KW - Electron-microscopy

KW - Flocculation

KW - Schizosaccharomyces pombe

UR - http://www.scopus.com/inward/record.url?scp=34250658025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250658025&partnerID=8YFLogxK

U2 - 10.1556/AMicr.54.2007.1.4

DO - 10.1556/AMicr.54.2007.1.4

M3 - Article

C2 - 17523390

AN - SCOPUS:34250658025

VL - 54

SP - 35

EP - 46

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 1

ER -