Cell trivision of hyperploid cells

Gabor Nagy, Gabor Kiraly, Melinda Turani, G. Bánfalvi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Malignant transformation is likely to render cells hyperploid, primarily tetraploid. We have measured the frequency of division into three rather than two daughter cells as a function of ploidy. Such trivisions were followed in near-tetraploid uveal melanoma (UM), hypotetraploid HaCaT (3 N), and in near-diploid (∼2 N) lung epithelial cell lines by time-lapse image analyses. A stepwise analysis of cytokinesis revealed higher frequency of cell trivisions relative to divisions in hyperploid HeLa (1:24, 4%), HaCaT (1:126, 8%), and UM (1:186, 0.5%) cells. The occurrence of trivision was significantly lower in near-diploid endothelial cells (1:1400, 0.07%). We have previously observed the phenomenon of trivision in HaCaT cells treated with heavy metal lead, and here we describe that trivision is a spontaneous process taking place without genotoxic treatment. Beside re-diploidization by trivision, the hyperploid state decreases the cell size of the daughter cells and is likely to increase the time of cytokinesis. On the basis of the results, it is hypothesized that among other cancer-related causes, hyperploidy could be related to cell trivision, could cause random aneuploidy, and could generate new cancer-specific karyotypes.

Original languageEnglish
Pages (from-to)676-684
Number of pages9
JournalDNA and Cell Biology
Volume32
Issue number12
DOIs
Publication statusPublished - Dec 1 2013

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Cytokinesis
Tetraploidy
Diploidy
Ploidies
Aneuploidy
Heavy Metals
Karyotype
Cell Size
Neoplasms
Endothelial Cells
Epithelial Cells
Cell Line
Lung
Uveal melanoma
Lead

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology
  • Medicine(all)

Cite this

Cell trivision of hyperploid cells. / Nagy, Gabor; Kiraly, Gabor; Turani, Melinda; Bánfalvi, G.

In: DNA and Cell Biology, Vol. 32, No. 12, 01.12.2013, p. 676-684.

Research output: Contribution to journalArticle

Nagy, G, Kiraly, G, Turani, M & Bánfalvi, G 2013, 'Cell trivision of hyperploid cells', DNA and Cell Biology, vol. 32, no. 12, pp. 676-684. https://doi.org/10.1089/dna.2013.2147
Nagy, Gabor ; Kiraly, Gabor ; Turani, Melinda ; Bánfalvi, G. / Cell trivision of hyperploid cells. In: DNA and Cell Biology. 2013 ; Vol. 32, No. 12. pp. 676-684.
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