Cell production and morphological pattern formation in primary brain cell cultures. I. Pattern formation within the basal layer(s)

E. Madarász, J. Kiss, I. Bartok

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Spontaneous pattern formation within the basal cellular layer of primary brain cell cultures was studied. Basal cells were found to be organized into a limited number of morphologically well distinguishable types of cell-arrangements. Phase contrast microscopy was used to characterize the different cell-assemblies morphologically. Under standard culture conditions the time of appearance and specific changes of relative frequency in time were also characteristics of different patterns. The distribution of mitotically active cells among the different morphological patterns within the basal layer was also investigated by recording [3H]thymidine uptake of cells. Reduction of mitotic activities of cells in the basal layer was found in the vicinity of overlying cells. In a given area of cultures the reduction of mitotic activity was proportional to the number of overlying cells. The influence of cell proliferation on the morphological pattern formation in primary cultures is discussed.

Original languageEnglish
Pages (from-to)339-349
Number of pages11
JournalBrain Research
Volume304
Issue number2
DOIs
Publication statusPublished - Jun 25 1984

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Primary Cell Culture
Brain
Phase-Contrast Microscopy
Thymidine
Cell Count
Cell Proliferation

Keywords

  • mitotic activity
  • morphological pattern formation
  • primary brain cell cultures

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Cell production and morphological pattern formation in primary brain cell cultures. I. Pattern formation within the basal layer(s). / Madarász, E.; Kiss, J.; Bartok, I.

In: Brain Research, Vol. 304, No. 2, 25.06.1984, p. 339-349.

Research output: Contribution to journalArticle

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