Cell-penetrating peptides and protein transduction domains in drug delivery

E. Duda, Tamás Letoha

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

High molecular weight biomolecules are becoming increasingly important for the development of molecular therapies. However, these molecules cannot diffuse through the cell membrane the way traditional small molecule drugs can. The potential therapeutic use of such information-rich macromolecules has been limited by their poor permeability across the lipid bilayer of biological membranes. Different approaches have been used to develop biological agents, biophysical methods or mimicking natural processes to overcome the barrier function of the plasma membrane. The unique capability of special oligopeptides, known as cellpenetrating peptides (CPPs) or protein transduction domains (PTDs), opened the way to introducing biologically active macromolecules into living cells. These peptides, conjugated or complexed with the desired molecules, can be used in both in vitro and in vivo systems for the intracellular or intraorganellar delivery of large molecules. After the discovery of these small peptides, macromolecular transport across biological membranes has emerged as a valuable technique in basic research and a promising tool in in vivo preclinical models and clinical trials. This chapter attempts to give an overview of what kind of CPPs exist, how they function, what has been achieved and what can be achieved in the future with their use. It also aims to expose the limitations of the utilization of CPPs and the controversies regarding their potential, working mechanism and route of internalization. Finally, we want to discuss how virus-, liposome- and CPP-mediated techniques converge into new complex methodologies.

Original languageEnglish
Title of host publicationSolubility, Delivery and ADME Problems of Drugs and Drug-Candidates
PublisherBentham Science Publishers Ltd.
Pages189-212
Number of pages24
ISBN (Print)9781608056194
DOIs
Publication statusPublished - 2011

Fingerprint

Cell-Penetrating Peptides
Peptides
Pharmaceutical Preparations
Biological Transport
Cell Membrane
Oligopeptides
Membranes
Biological Factors
Lipid Bilayers
Therapeutic Uses
Liposomes
Protein Domains
Permeability
Molecular Weight
Clinical Trials
Viruses
Research

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Duda, E., & Letoha, T. (2011). Cell-penetrating peptides and protein transduction domains in drug delivery. In Solubility, Delivery and ADME Problems of Drugs and Drug-Candidates (pp. 189-212). Bentham Science Publishers Ltd.. https://doi.org/10.2174/978160805120511101010189

Cell-penetrating peptides and protein transduction domains in drug delivery. / Duda, E.; Letoha, Tamás.

Solubility, Delivery and ADME Problems of Drugs and Drug-Candidates. Bentham Science Publishers Ltd., 2011. p. 189-212.

Research output: Chapter in Book/Report/Conference proceedingChapter

Duda, E & Letoha, T 2011, Cell-penetrating peptides and protein transduction domains in drug delivery. in Solubility, Delivery and ADME Problems of Drugs and Drug-Candidates. Bentham Science Publishers Ltd., pp. 189-212. https://doi.org/10.2174/978160805120511101010189
Duda E, Letoha T. Cell-penetrating peptides and protein transduction domains in drug delivery. In Solubility, Delivery and ADME Problems of Drugs and Drug-Candidates. Bentham Science Publishers Ltd. 2011. p. 189-212 https://doi.org/10.2174/978160805120511101010189
Duda, E. ; Letoha, Tamás. / Cell-penetrating peptides and protein transduction domains in drug delivery. Solubility, Delivery and ADME Problems of Drugs and Drug-Candidates. Bentham Science Publishers Ltd., 2011. pp. 189-212
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