Cell migration or cytokinesis and proliferation? - Revisiting the "go or grow" hypothesis in cancer cells in vitro

Tamás Garay, Éva Juhász, Eszter Molnár, Maria Eisenbauer, A. Czirók, Barbara Dekan, Viktória László, Mir Alireza Hoda, B. Döme, J. Tímár, Walter Klepetko, Walter Berger, B. Hegedűs

Research output: Contribution to journalArticle

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Abstract

The mortality of patients with solid tumors is mostly due to metastasis that relies on the interplay between migration and proliferation. The "go or grow" hypothesis postulates that migration and proliferation spatiotemporally excludes each other.We evaluated this hypothesis on 35 cell lines (12 mesothelioma, 13 melanoma and 10 lung cancer) on both the individual cell and population levels. Following three-day-long videomicroscopy, migration, proliferation and cytokinesis-length were quantified. We found a significantly higher migration in mesothelioma cells compared to melanoma and lung cancer while tumor types did not differ in mean proliferation or duration of cytokinesis. Strikingly, we found in melanoma and lung cancer a significant positive correlation between mean proliferation and migration. Furthermore, non-dividing melanoma and lung cancer cells displayed slower migration. In contrast, in mesothelioma there were no such correlations. Interestingly, negative correlation was found between cytokinesis-length and migration in melanoma. FAK activation was higher in melanoma cells with high motility.We demonstrate that the cancer cells studied do not defer proliferation for migration. Of note, tumor cells from various organ systems may differently regulate migration and proliferation. Furthermore, our data is in line with the observation of pathologists that highly proliferative tumors are often highly invasive.

Original languageEnglish
Pages (from-to)3094-3103
Number of pages10
JournalExperimental Cell Research
Volume319
Issue number20
DOIs
Publication statusPublished - Dec 10 2013

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Cytokinesis
Cell Movement
Melanoma
Lung Neoplasms
Mesothelioma
Neoplasms
Video Microscopy
In Vitro Techniques
Observation
Neoplasm Metastasis
Cell Line
Mortality
Population

Keywords

  • Cytokinesis
  • Lung cancer
  • Melanoma
  • Mesothelioma
  • Migration
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cell migration or cytokinesis and proliferation? - Revisiting the "go or grow" hypothesis in cancer cells in vitro. / Garay, Tamás; Juhász, Éva; Molnár, Eszter; Eisenbauer, Maria; Czirók, A.; Dekan, Barbara; László, Viktória; Hoda, Mir Alireza; Döme, B.; Tímár, J.; Klepetko, Walter; Berger, Walter; Hegedűs, B.

In: Experimental Cell Research, Vol. 319, No. 20, 10.12.2013, p. 3094-3103.

Research output: Contribution to journalArticle

Garay, Tamás ; Juhász, Éva ; Molnár, Eszter ; Eisenbauer, Maria ; Czirók, A. ; Dekan, Barbara ; László, Viktória ; Hoda, Mir Alireza ; Döme, B. ; Tímár, J. ; Klepetko, Walter ; Berger, Walter ; Hegedűs, B. / Cell migration or cytokinesis and proliferation? - Revisiting the "go or grow" hypothesis in cancer cells in vitro. In: Experimental Cell Research. 2013 ; Vol. 319, No. 20. pp. 3094-3103.
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