Cell-mediated immune responses to influenza vaccination in healthy volunteers and allogeneic stem cell transplant recipients

G. Avetisyan, E. Ragnavölgyi, G. Tóth, M. Hassan, Per Ljungman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Influenza is one of the most common respiratory diseases in humans. The response to vaccination is frequently poor in immunosuppressed individuals. The aim of the present study was to develop an enzyme-linked immunospot (ELISPOT) assay for measuring of the specific T-cell response to influenza vaccination. In all, 18 healthy subjects and six stem cell transplantation (SCT) patients tested before and 4 weeks after influenza vaccination were included in the present study. Peripheral blood lymphocytes were stimulated with four influenza peptides; three based on sequences from the hemagglutinin and one from the M1 protein. The ELISPOT assay and the measurement of intracellular IFN-γ production were used to determine the cell-mediated responses after stimulation with the peptides. Influenza vaccination elicited strong cell-mediated immune responses in the healthy controls to all four peptides with 3.2-6.9-fold increases in the number of IFN-γ producing spots/106 cells. By intracellular staining, it was suggested that CD4+ cells mediated the responses to the hemagglutinin peptides. In contrast, there was no increase in the number of IFN-γ producing cells response after vaccination in the six SCT patients. In conclusion, our results suggest that the ELISPOT assay might be used as a complement to serology for monitoring of future influenza vaccine studies in SCT patients.

Original languageEnglish
Pages (from-to)411-415
Number of pages5
JournalBone Marrow Transplantation
Volume36
Issue number5
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Human Influenza
Healthy Volunteers
Vaccination
Stem Cells
Enzyme-Linked Immunospot Assay
Transplants
Stem Cell Transplantation
Peptides
Hemagglutinins
Influenza Vaccines
Serology
Transplant Recipients
Lymphocytes
Staining and Labeling
T-Lymphocytes
Proteins

Keywords

  • Cell-mediated immunity
  • Elispot
  • Influenza
  • Stem cell transplantation
  • Vaccination

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Cell-mediated immune responses to influenza vaccination in healthy volunteers and allogeneic stem cell transplant recipients. / Avetisyan, G.; Ragnavölgyi, E.; Tóth, G.; Hassan, M.; Ljungman, Per.

In: Bone Marrow Transplantation, Vol. 36, No. 5, 09.2005, p. 411-415.

Research output: Contribution to journalArticle

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