Cell free DNA of tumor origin induces a 'metastatic' expression profile in HT-29 cancer cell line

István Furi, Alexandra Kalmár, Barnabás Wichmann, S. Spisák, Andrea Schöller, Barbara Barták, Z. Tulassay, B. Molnár

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Epithelial cells in malignant conditions release DNA into the extracellular compartment. Cell free DNA of tumor origin may act as a ligand of DNA sensing mechanisms and mediate changes in epithelial-stromal interactions. Aims: To evaluate and compare the potential autocrine and paracrine regulatory effect of normal and malignant epithelial cell-related DNA on TLR9 and STING mediated pathways in HT-29 human colorectal adenocarcinoma cells and normal fibroblasts. Materials and Methods: DNA isolated from normal and tumorous colonic epithelia of fresh frozen surgically removed tissue samples was used for 24 and 6 hour treatment of HT-29 colon carcinoma and HDF-α fibroblast cells. Whole genome mRNA expression analysis and qRT-PCR was performed for the elements/members of TLR9 signaling pathway. Immunocytochemistry was performed for epithelial markers (i.e. CK20 and E-cadherin), DNA methyltransferase 3a (DNMT3a) and NFκB (for treated HDFα cells). Results: Administration of tumor derived DNA on HT29 cells resulted in significant (p

Original languageEnglish
Article numbere0131699
JournalPLoS One
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 2 2015

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HT29 Cells
Tumors
Cells
cell lines
Cell Line
neoplasms
DNA
Neoplasms
cells
Fibroblasts
fibroblasts
Epithelial Cells
epithelial cells
Cadherins
cadherins
methyltransferases
immunocytochemistry
adenocarcinoma
neoplasm cells
Colon

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cell free DNA of tumor origin induces a 'metastatic' expression profile in HT-29 cancer cell line. / Furi, István; Kalmár, Alexandra; Wichmann, Barnabás; Spisák, S.; Schöller, Andrea; Barták, Barbara; Tulassay, Z.; Molnár, B.

In: PLoS One, Vol. 10, No. 7, e0131699, 02.07.2015.

Research output: Contribution to journalArticle

Furi, István ; Kalmár, Alexandra ; Wichmann, Barnabás ; Spisák, S. ; Schöller, Andrea ; Barták, Barbara ; Tulassay, Z. ; Molnár, B. / Cell free DNA of tumor origin induces a 'metastatic' expression profile in HT-29 cancer cell line. In: PLoS One. 2015 ; Vol. 10, No. 7.
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AU - Wichmann, Barnabás

AU - Spisák, S.

AU - Schöller, Andrea

AU - Barták, Barbara

AU - Tulassay, Z.

AU - Molnár, B.

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