Cell differentiation of the fetal rat anterior pituitary in vitro

Ágnes Nemeskéry, Anna Németh, Gy Sétáló, S. Vigh, B. Halász

Research output: Contribution to journalArticle

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Abstract

Adenohypophysial primordia of rat embryos at 13 to 15 days gestation were cultured in Parker 199 synthetic medium for 2 to 11 days. At the end of the culture period their fine structure and the presence of immunoreactive trophic hormones using the peroxidase-labeled antibody technique were investigated. The degree of differentiation in the glands depends largely on the age of the embryos furnishing the explants. Cultured pituitaries explanted on the 13th day of gestation contain only ACTH-positive cells and about 15% of the cells are granular. The granules are 50-100 nm in diameter in some cells, while in other cells they range from 50 to 200 nm. In cultivated adenohypophysial primordia of embryos on the 15th day of intrauterine life ACTH, prolactin, LH and TSH cells are evident, but only the same two kinds of granular cells can be observed with the electron microscope. The extent of cytodifferentiation in the glands explanted on the 14th day of gestation is intermediate between the two other groups. The data suggest that the fetal rat pituitary has the capacity of self-differentiation but to a lesser extent than that of the in situ hypophysis.

Original languageEnglish
Pages (from-to)263-273
Number of pages11
JournalCell and Tissue Research
Volume170
Issue number2
DOIs
Publication statusPublished - Jul 1976

Fingerprint

Adrenocorticotropic Hormone
Rats
Cell Differentiation
Prolactin
Embryonic Structures
Peroxidase
Electron microscopes
Pregnancy
Cells
Hormones
Antibodies
Thyrotrophs
Gonadotrophs
Pituitary Gland
Immunoenzyme Techniques
In Vitro Techniques
Electrons

Keywords

  • Cell differentiation
  • Fetal pituitary
  • Immunocytochemistry
  • Tissue culture

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Cell differentiation of the fetal rat anterior pituitary in vitro. / Nemeskéry, Ágnes; Németh, Anna; Sétáló, Gy; Vigh, S.; Halász, B.

In: Cell and Tissue Research, Vol. 170, No. 2, 07.1976, p. 263-273.

Research output: Contribution to journalArticle

Nemeskéry, Á, Németh, A, Sétáló, G, Vigh, S & Halász, B 1976, 'Cell differentiation of the fetal rat anterior pituitary in vitro', Cell and Tissue Research, vol. 170, no. 2, pp. 263-273. https://doi.org/10.1007/BF00224303
Nemeskéry, Ágnes ; Németh, Anna ; Sétáló, Gy ; Vigh, S. ; Halász, B. / Cell differentiation of the fetal rat anterior pituitary in vitro. In: Cell and Tissue Research. 1976 ; Vol. 170, No. 2. pp. 263-273.
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