After a few days cultivation some of the pituitary cells became granulated. This cell differentiation largely depends on the age of the embryo when the hypophysis was explanted. In pituitaries removed on the 13th day of gestation about 15% of the cells were granular and contained a small number of membrane bound granules. In the pituitaries from the 14th day of gestation about 40%, in those from the 15 day old ones 60% of the cells became granulated by the end of the 7 days' culture period. Hypothalamus or cortex extract or synthetic LH RH, TRH or somatostatin when added to the incubation medium had no appreciable effect on cell differentiation of the cultured fetal rat pituitaries examined with the electron microscope and by immunohistochemical approach.
|Number of pages||7|
|Publication status||Published - Jan 1 1976|
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