Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract

Andrea Krisztina Sükösd, Judit Rapp, Diána Feller, György Sétáló, Beáta Gáspár, J. Pongrácz, H. Ábrahám, Zsolt Biró

Research output: Contribution to journalArticle

Abstract

AIM: To study molecular and morphological changes in lens epithelial cells following femtosecond laser-assisted and manually performed continuous curvilinear capsulotomy (CCC) in order to get information about these methods regarding their potential role in the induction of development of secondary cataract. ● METHODS: Anterior lens capsules (ALC) were removed from 40 patients with age-related cataract by manual CCC and by femtosecond laser-assisted capsulotomy (FLAC). Samples removed by manual CCC were assorted in group 1, FLAC samples were classified in group 2. Morphology of lens epithelial cells was examined with light and electron microscopes. Following capsulotomy, expressions of p53, Bcl-2 and cyclin D1 genes were analyzed with reverse transcriptase polymerase chain reaction. Immunohistochemistry was used to detect the pro-apoptotic p53 in the epithelial cells. ● RESULTS: Light and electron microscopic examination showed that ALC of group 1 contained more degenerating cells following manual CCC than after FLAC. The expression level of p53 was higher after manual than laser-assisted surgery. Immunocytochemistry indicated significantly higher number of cells containing p53 protein in the manual CCC group than following FLAC. Bcl-2 and cyclin D1 gene expression levels were slightly lower following manual CCC than after FLAC, but the difference was not significant. ● CONCLUSION: Manually removed ALC shows slightly, but not significantly larger damage due to the mechanical stretching and pulling of the capsule than those removed using FLAC.

Original languageEnglish
Pages (from-to)1440-1446
Number of pages7
JournalInternational Journal of Ophthalmology
Volume11
Issue number9
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Cataract
Cell Survival
Lasers
Cell Death
Anterior Capsule of the Lens
bcl-1 Genes
Epithelial Cells
Lenses
Immunohistochemistry
Electrons
Capsule Opacification
Light
Laser Therapy
Reverse Transcriptase Polymerase Chain Reaction
Capsules
Cell Count
Gene Expression
Proteins

Keywords

  • Capsulorhexis
  • Capsulotomy
  • Femtosecond laser
  • Gene expression
  • Immunhistology
  • Ultrastructure

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract. / Sükösd, Andrea Krisztina; Rapp, Judit; Feller, Diána; Sétáló, György; Gáspár, Beáta; Pongrácz, J.; Ábrahám, H.; Biró, Zsolt.

In: International Journal of Ophthalmology, Vol. 11, No. 9, 01.01.2018, p. 1440-1446.

Research output: Contribution to journalArticle

Sükösd, Andrea Krisztina ; Rapp, Judit ; Feller, Diána ; Sétáló, György ; Gáspár, Beáta ; Pongrácz, J. ; Ábrahám, H. ; Biró, Zsolt. / Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract. In: International Journal of Ophthalmology. 2018 ; Vol. 11, No. 9. pp. 1440-1446.
@article{3f416214c16c4948a0a86520bf34439a,
title = "Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract",
abstract = "AIM: To study molecular and morphological changes in lens epithelial cells following femtosecond laser-assisted and manually performed continuous curvilinear capsulotomy (CCC) in order to get information about these methods regarding their potential role in the induction of development of secondary cataract. ● METHODS: Anterior lens capsules (ALC) were removed from 40 patients with age-related cataract by manual CCC and by femtosecond laser-assisted capsulotomy (FLAC). Samples removed by manual CCC were assorted in group 1, FLAC samples were classified in group 2. Morphology of lens epithelial cells was examined with light and electron microscopes. Following capsulotomy, expressions of p53, Bcl-2 and cyclin D1 genes were analyzed with reverse transcriptase polymerase chain reaction. Immunohistochemistry was used to detect the pro-apoptotic p53 in the epithelial cells. ● RESULTS: Light and electron microscopic examination showed that ALC of group 1 contained more degenerating cells following manual CCC than after FLAC. The expression level of p53 was higher after manual than laser-assisted surgery. Immunocytochemistry indicated significantly higher number of cells containing p53 protein in the manual CCC group than following FLAC. Bcl-2 and cyclin D1 gene expression levels were slightly lower following manual CCC than after FLAC, but the difference was not significant. ● CONCLUSION: Manually removed ALC shows slightly, but not significantly larger damage due to the mechanical stretching and pulling of the capsule than those removed using FLAC.",
keywords = "Capsulorhexis, Capsulotomy, Femtosecond laser, Gene expression, Immunhistology, Ultrastructure",
author = "S{\"u}k{\"o}sd, {Andrea Krisztina} and Judit Rapp and Di{\'a}na Feller and Gy{\"o}rgy S{\'e}t{\'a}l{\'o} and Be{\'a}ta G{\'a}sp{\'a}r and J. Pongr{\'a}cz and H. {\'A}brah{\'a}m and Zsolt Bir{\'o}",
year = "2018",
month = "1",
day = "1",
doi = "10.18240/ijo.2018.09.02",
language = "English",
volume = "11",
pages = "1440--1446",
journal = "International Journal of Ophthalmology",
issn = "2222-3959",
publisher = "Press of International Journal of Ophthalmology",
number = "9",

}

TY - JOUR

T1 - Cell death and survival following manual and femtosecond laser-assisted capsulotomy in age-related cataract

AU - Sükösd, Andrea Krisztina

AU - Rapp, Judit

AU - Feller, Diána

AU - Sétáló, György

AU - Gáspár, Beáta

AU - Pongrácz, J.

AU - Ábrahám, H.

AU - Biró, Zsolt

PY - 2018/1/1

Y1 - 2018/1/1

N2 - AIM: To study molecular and morphological changes in lens epithelial cells following femtosecond laser-assisted and manually performed continuous curvilinear capsulotomy (CCC) in order to get information about these methods regarding their potential role in the induction of development of secondary cataract. ● METHODS: Anterior lens capsules (ALC) were removed from 40 patients with age-related cataract by manual CCC and by femtosecond laser-assisted capsulotomy (FLAC). Samples removed by manual CCC were assorted in group 1, FLAC samples were classified in group 2. Morphology of lens epithelial cells was examined with light and electron microscopes. Following capsulotomy, expressions of p53, Bcl-2 and cyclin D1 genes were analyzed with reverse transcriptase polymerase chain reaction. Immunohistochemistry was used to detect the pro-apoptotic p53 in the epithelial cells. ● RESULTS: Light and electron microscopic examination showed that ALC of group 1 contained more degenerating cells following manual CCC than after FLAC. The expression level of p53 was higher after manual than laser-assisted surgery. Immunocytochemistry indicated significantly higher number of cells containing p53 protein in the manual CCC group than following FLAC. Bcl-2 and cyclin D1 gene expression levels were slightly lower following manual CCC than after FLAC, but the difference was not significant. ● CONCLUSION: Manually removed ALC shows slightly, but not significantly larger damage due to the mechanical stretching and pulling of the capsule than those removed using FLAC.

AB - AIM: To study molecular and morphological changes in lens epithelial cells following femtosecond laser-assisted and manually performed continuous curvilinear capsulotomy (CCC) in order to get information about these methods regarding their potential role in the induction of development of secondary cataract. ● METHODS: Anterior lens capsules (ALC) were removed from 40 patients with age-related cataract by manual CCC and by femtosecond laser-assisted capsulotomy (FLAC). Samples removed by manual CCC were assorted in group 1, FLAC samples were classified in group 2. Morphology of lens epithelial cells was examined with light and electron microscopes. Following capsulotomy, expressions of p53, Bcl-2 and cyclin D1 genes were analyzed with reverse transcriptase polymerase chain reaction. Immunohistochemistry was used to detect the pro-apoptotic p53 in the epithelial cells. ● RESULTS: Light and electron microscopic examination showed that ALC of group 1 contained more degenerating cells following manual CCC than after FLAC. The expression level of p53 was higher after manual than laser-assisted surgery. Immunocytochemistry indicated significantly higher number of cells containing p53 protein in the manual CCC group than following FLAC. Bcl-2 and cyclin D1 gene expression levels were slightly lower following manual CCC than after FLAC, but the difference was not significant. ● CONCLUSION: Manually removed ALC shows slightly, but not significantly larger damage due to the mechanical stretching and pulling of the capsule than those removed using FLAC.

KW - Capsulorhexis

KW - Capsulotomy

KW - Femtosecond laser

KW - Gene expression

KW - Immunhistology

KW - Ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=85053375044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053375044&partnerID=8YFLogxK

U2 - 10.18240/ijo.2018.09.02

DO - 10.18240/ijo.2018.09.02

M3 - Article

VL - 11

SP - 1440

EP - 1446

JO - International Journal of Ophthalmology

JF - International Journal of Ophthalmology

SN - 2222-3959

IS - 9

ER -