Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells

Z. Magyar, T. Mészáros, Pál Miskolczi, Mária Deák, A. Fehér, Spencer Brown, É. Kondorosi, Alekos Athanasiadis, Sándor Pongor, Metin Bilgin, László Bakó, Csaba Koncz, D. Dudits

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

The eukaryotic cell division cycle is coordinated by cyclin-dependent kinases (CDKs), represented by a single major serine/threonine kinase in yeasts (Cdc2/CDC28) and a family of kinases (CDK1 to CDK8) in human cells. Previously, two cdc2 homologs, cdc2MsA and cdc2MsB, have been identified in alfalfa (Medicago sativa). By isolating cDNAs using a cdc2MsA probe, we demonstrate here that at least four additional cdc2 homologous genes are expressed in the tetraploid alfalfa. Proteins encoded by the new cdc2MsC to cdc2MsF cDNAs share the characteristic functional domains of CDKs with the conserved and plant-specific sequence elements. Transcripts from cdc2MsA, cdc2MsB, cdc2MsC, and cdc2MsE genes are synthesized throughout the cell cycle, whereas the amounts of cdc2MsD and cdc2MsF mRNAs peak during G2-to-M phases. The translation of Cdc2MsA/B, Cdc2MsD, and Cdc2MsF proteins follows the pattern of transcript accumulation. The multiplicity of kinase complexes with cell cycle phase-dependent activities was revealed by in vitro phosphorylation experiments. Proteins bound to p13(suc1)-Sepharose or immunoprecipitated with Cdc2MsA/B antibodies from cells at G1-to-S and G2-to-M phase boundaries showed elevated kinase activities. The Cdc2MsF antibodies separated a G2-to-M phase-related kinase complex. Detection of histone H1 phosphorylation activities in fractions immunoprecipitated with antimitotic cyclin (CyclinMs2) antibodies from G2-to-M phase cells indicates the complex formation between this cyclin and a kinase partner in alfalfa. The observed fluctuation of transcript levels, amounts, and activities of kinases in different cell cycle phases reflects a multilevel regulatory system during cell cycle progression in plants.

Original languageEnglish
Pages (from-to)223-235
Number of pages13
JournalPlant Cell
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 1997

Fingerprint

Medicago sativa
alfalfa
cell cycle
Cell Cycle
phosphotransferases (kinases)
Phosphotransferases
Cells
Cell Division
mitosis
cells
Phosphorylation
Antibodies
antibodies
Complementary DNA
Genes
phosphorylation
Antimitotic Agents
Proteins
Tetraploidy
Cyclin-Dependent Kinases

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells. / Magyar, Z.; Mészáros, T.; Miskolczi, Pál; Deák, Mária; Fehér, A.; Brown, Spencer; Kondorosi, É.; Athanasiadis, Alekos; Pongor, Sándor; Bilgin, Metin; Bakó, László; Koncz, Csaba; Dudits, D.

In: Plant Cell, Vol. 9, No. 2, 02.1997, p. 223-235.

Research output: Contribution to journalArticle

Magyar, Z. ; Mészáros, T. ; Miskolczi, Pál ; Deák, Mária ; Fehér, A. ; Brown, Spencer ; Kondorosi, É. ; Athanasiadis, Alekos ; Pongor, Sándor ; Bilgin, Metin ; Bakó, László ; Koncz, Csaba ; Dudits, D. / Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells. In: Plant Cell. 1997 ; Vol. 9, No. 2. pp. 223-235.
@article{a5e9829af32544caa6c01cb2437cfd6f,
title = "Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells",
abstract = "The eukaryotic cell division cycle is coordinated by cyclin-dependent kinases (CDKs), represented by a single major serine/threonine kinase in yeasts (Cdc2/CDC28) and a family of kinases (CDK1 to CDK8) in human cells. Previously, two cdc2 homologs, cdc2MsA and cdc2MsB, have been identified in alfalfa (Medicago sativa). By isolating cDNAs using a cdc2MsA probe, we demonstrate here that at least four additional cdc2 homologous genes are expressed in the tetraploid alfalfa. Proteins encoded by the new cdc2MsC to cdc2MsF cDNAs share the characteristic functional domains of CDKs with the conserved and plant-specific sequence elements. Transcripts from cdc2MsA, cdc2MsB, cdc2MsC, and cdc2MsE genes are synthesized throughout the cell cycle, whereas the amounts of cdc2MsD and cdc2MsF mRNAs peak during G2-to-M phases. The translation of Cdc2MsA/B, Cdc2MsD, and Cdc2MsF proteins follows the pattern of transcript accumulation. The multiplicity of kinase complexes with cell cycle phase-dependent activities was revealed by in vitro phosphorylation experiments. Proteins bound to p13(suc1)-Sepharose or immunoprecipitated with Cdc2MsA/B antibodies from cells at G1-to-S and G2-to-M phase boundaries showed elevated kinase activities. The Cdc2MsF antibodies separated a G2-to-M phase-related kinase complex. Detection of histone H1 phosphorylation activities in fractions immunoprecipitated with antimitotic cyclin (CyclinMs2) antibodies from G2-to-M phase cells indicates the complex formation between this cyclin and a kinase partner in alfalfa. The observed fluctuation of transcript levels, amounts, and activities of kinases in different cell cycle phases reflects a multilevel regulatory system during cell cycle progression in plants.",
author = "Z. Magyar and T. M{\'e}sz{\'a}ros and P{\'a}l Miskolczi and M{\'a}ria De{\'a}k and A. Feh{\'e}r and Spencer Brown and {\'E}. Kondorosi and Alekos Athanasiadis and S{\'a}ndor Pongor and Metin Bilgin and L{\'a}szl{\'o} Bak{\'o} and Csaba Koncz and D. Dudits",
year = "1997",
month = "2",
doi = "10.1105/tpc.9.2.223",
language = "English",
volume = "9",
pages = "223--235",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "2",

}

TY - JOUR

T1 - Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells

AU - Magyar, Z.

AU - Mészáros, T.

AU - Miskolczi, Pál

AU - Deák, Mária

AU - Fehér, A.

AU - Brown, Spencer

AU - Kondorosi, É.

AU - Athanasiadis, Alekos

AU - Pongor, Sándor

AU - Bilgin, Metin

AU - Bakó, László

AU - Koncz, Csaba

AU - Dudits, D.

PY - 1997/2

Y1 - 1997/2

N2 - The eukaryotic cell division cycle is coordinated by cyclin-dependent kinases (CDKs), represented by a single major serine/threonine kinase in yeasts (Cdc2/CDC28) and a family of kinases (CDK1 to CDK8) in human cells. Previously, two cdc2 homologs, cdc2MsA and cdc2MsB, have been identified in alfalfa (Medicago sativa). By isolating cDNAs using a cdc2MsA probe, we demonstrate here that at least four additional cdc2 homologous genes are expressed in the tetraploid alfalfa. Proteins encoded by the new cdc2MsC to cdc2MsF cDNAs share the characteristic functional domains of CDKs with the conserved and plant-specific sequence elements. Transcripts from cdc2MsA, cdc2MsB, cdc2MsC, and cdc2MsE genes are synthesized throughout the cell cycle, whereas the amounts of cdc2MsD and cdc2MsF mRNAs peak during G2-to-M phases. The translation of Cdc2MsA/B, Cdc2MsD, and Cdc2MsF proteins follows the pattern of transcript accumulation. The multiplicity of kinase complexes with cell cycle phase-dependent activities was revealed by in vitro phosphorylation experiments. Proteins bound to p13(suc1)-Sepharose or immunoprecipitated with Cdc2MsA/B antibodies from cells at G1-to-S and G2-to-M phase boundaries showed elevated kinase activities. The Cdc2MsF antibodies separated a G2-to-M phase-related kinase complex. Detection of histone H1 phosphorylation activities in fractions immunoprecipitated with antimitotic cyclin (CyclinMs2) antibodies from G2-to-M phase cells indicates the complex formation between this cyclin and a kinase partner in alfalfa. The observed fluctuation of transcript levels, amounts, and activities of kinases in different cell cycle phases reflects a multilevel regulatory system during cell cycle progression in plants.

AB - The eukaryotic cell division cycle is coordinated by cyclin-dependent kinases (CDKs), represented by a single major serine/threonine kinase in yeasts (Cdc2/CDC28) and a family of kinases (CDK1 to CDK8) in human cells. Previously, two cdc2 homologs, cdc2MsA and cdc2MsB, have been identified in alfalfa (Medicago sativa). By isolating cDNAs using a cdc2MsA probe, we demonstrate here that at least four additional cdc2 homologous genes are expressed in the tetraploid alfalfa. Proteins encoded by the new cdc2MsC to cdc2MsF cDNAs share the characteristic functional domains of CDKs with the conserved and plant-specific sequence elements. Transcripts from cdc2MsA, cdc2MsB, cdc2MsC, and cdc2MsE genes are synthesized throughout the cell cycle, whereas the amounts of cdc2MsD and cdc2MsF mRNAs peak during G2-to-M phases. The translation of Cdc2MsA/B, Cdc2MsD, and Cdc2MsF proteins follows the pattern of transcript accumulation. The multiplicity of kinase complexes with cell cycle phase-dependent activities was revealed by in vitro phosphorylation experiments. Proteins bound to p13(suc1)-Sepharose or immunoprecipitated with Cdc2MsA/B antibodies from cells at G1-to-S and G2-to-M phase boundaries showed elevated kinase activities. The Cdc2MsF antibodies separated a G2-to-M phase-related kinase complex. Detection of histone H1 phosphorylation activities in fractions immunoprecipitated with antimitotic cyclin (CyclinMs2) antibodies from G2-to-M phase cells indicates the complex formation between this cyclin and a kinase partner in alfalfa. The observed fluctuation of transcript levels, amounts, and activities of kinases in different cell cycle phases reflects a multilevel regulatory system during cell cycle progression in plants.

UR - http://www.scopus.com/inward/record.url?scp=1842288563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842288563&partnerID=8YFLogxK

U2 - 10.1105/tpc.9.2.223

DO - 10.1105/tpc.9.2.223

M3 - Article

C2 - 9061953

AN - SCOPUS:1842288563

VL - 9

SP - 223

EP - 235

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 2

ER -