Cell cycle analysis can differentiate thin melanomas from dysplastic nevi and reveals accelerated replication in thick melanomas

Gergo Kiszner, Barnabas Wichmann, Istvan B. Nemeth, Erika Varga, Nora Meggyeshazi, Ivett Teleki, Peter Balla, Mate E. Maros, Karoly Penksza, T. Krenács

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cell replication integrates aberrations of cell cycle regulation and diverse upstream pathways which all can contribute to melanoma development and progression. In this study, cell cycle regulatory proteins were detected in situ in benign and malignant melanocytic tumors to allow correlation of major cell cycle fractions (G1, S-G2, and G2-M) with melanoma evolution. Dysplastic nevi expressed early cell cycle markers (cyclin D1 and cyclin-dependent kinase 2; Cdk2) significantly more (p1 mm) than in thin melanomas. Marker expression did not differ between metastatic melanomas and thick melanomas, with the exception of aurora kinase A of which the expression was higher in metastatic melanomas. Combined detection of cyclin A (post-G1 phase) with Mcm6 (replication licensing) and Ki67 correctly classified thin melanomas and dysplastic nevi in 95.9 % of the original samples and in 93.2 % of cross-validated grouped cases at 89.5 % sensitivity and 92.6 % specificity. Therefore, cell cycle phase marker detection can indicate malignancy in early melanocytic lesions and accelerated cell cycle progression during vertical melanoma growth.

Original languageEnglish
Pages (from-to)603-612
Number of pages10
JournalVirchows Archiv
Volume464
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Dysplastic Nevus Syndrome
Melanoma
Cell Cycle
Aurora Kinase A
Cyclin-Dependent Kinase 2
Cyclin A
Cell Cycle Proteins
Cyclin D1
G1 Phase
Licensure
Neoplasms
Sensitivity and Specificity

Keywords

  • Cell cycle phase analysis
  • Cutaneous melanoma
  • Ki67
  • Mcm6
  • Post-G1 phase markers

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

Cell cycle analysis can differentiate thin melanomas from dysplastic nevi and reveals accelerated replication in thick melanomas. / Kiszner, Gergo; Wichmann, Barnabas; Nemeth, Istvan B.; Varga, Erika; Meggyeshazi, Nora; Teleki, Ivett; Balla, Peter; Maros, Mate E.; Penksza, Karoly; Krenács, T.

In: Virchows Archiv, Vol. 464, No. 5, 2014, p. 603-612.

Research output: Contribution to journalArticle

Kiszner, G, Wichmann, B, Nemeth, IB, Varga, E, Meggyeshazi, N, Teleki, I, Balla, P, Maros, ME, Penksza, K & Krenács, T 2014, 'Cell cycle analysis can differentiate thin melanomas from dysplastic nevi and reveals accelerated replication in thick melanomas', Virchows Archiv, vol. 464, no. 5, pp. 603-612. https://doi.org/10.1007/s00428-014-1570-1
Kiszner, Gergo ; Wichmann, Barnabas ; Nemeth, Istvan B. ; Varga, Erika ; Meggyeshazi, Nora ; Teleki, Ivett ; Balla, Peter ; Maros, Mate E. ; Penksza, Karoly ; Krenács, T. / Cell cycle analysis can differentiate thin melanomas from dysplastic nevi and reveals accelerated replication in thick melanomas. In: Virchows Archiv. 2014 ; Vol. 464, No. 5. pp. 603-612.
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