Cell culture density dependent toxicity and chromatin changes upon cadmium treatment in murine pre-B-cells

G. Bánfalvi, Kinga Ujvarosi, Gyorgy Trencsenyi, Csilla Somogyi, Gabor Nagy, Alexei Basnakian

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Murine pre-B-cells grown in the presence of lower (1 μM) or higher (5 μM) concentration of cadmium chloride were separated into 13 fractions by centrifugal elutriation. The rate of DNA synthesis after cadmium treatment determined in permeable cells was dependent on cell culture density during cadmium treatment. Cell cycle analysis revealed a shift in the profile of DNA synthesis from replicative to repair DNA synthesis upon cadmium treatment. The study of the relationship between cell culture density and cell diameter at lower and higher cell densities in the presence of 1 μM cadmium chloride concentration showed that a. at 5×105 cell/ml or lower densities cells were shrinking indicating apoptotic changes, b. at higher cell culture densities the average cell size increased, c. the treatment of cells with low CdCl2 concentration (1 μM) at higher cell culture density (>5×105 cell/ml) did not change significantly the average cell diameter. At 5 μM cadmium concentration and higher cell culture densities (>5×105 cell/ml) the average cell size decreased in each elutriated fraction. Most significant inhibition of cell growth took place in early S phase (2.0-2.5 C value). Apoptotic chromatin changes in chromatin structure after cadmium treatment were seen as large extensive disruptions, holes in the nuclear membrane and stickiness of incompletely folded chromosomes.

Original languageEnglish
Pages (from-to)1219-1228
Number of pages10
JournalApoptosis
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 2007

Fingerprint

B-Lymphoid Precursor Cells
Cadmium
Cell culture
Chromatin
Toxicity
Cadmium Chloride
Cell Culture Techniques
Cell Count
Cells
DNA
Cell Size
Cell growth
Chromosomes
Repair
Nuclear Envelope
Membranes
S Phase
DNA Repair
Cell Cycle
Growth

Keywords

  • Apoptotic bodies
  • Cell cycle
  • DNA synthesis
  • Fluorescent microscopy
  • Interphase chromatin
  • Reversible permeabilization
  • Synchronization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Cell culture density dependent toxicity and chromatin changes upon cadmium treatment in murine pre-B-cells. / Bánfalvi, G.; Ujvarosi, Kinga; Trencsenyi, Gyorgy; Somogyi, Csilla; Nagy, Gabor; Basnakian, Alexei.

In: Apoptosis, Vol. 12, No. 7, 07.2007, p. 1219-1228.

Research output: Contribution to journalArticle

Bánfalvi, G. ; Ujvarosi, Kinga ; Trencsenyi, Gyorgy ; Somogyi, Csilla ; Nagy, Gabor ; Basnakian, Alexei. / Cell culture density dependent toxicity and chromatin changes upon cadmium treatment in murine pre-B-cells. In: Apoptosis. 2007 ; Vol. 12, No. 7. pp. 1219-1228.
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