Cell adhesion molecules in stromal corneal dystrophies

Gabor Nemeth, S. Felszeghy, Annamaria Kenyeres, Nora Szentmary, A. Berta, I. Süveges, L. Módis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present study was to investigate the expression pattern of different cell adhesion molecules in corneal stromal dystrophies. Fifteen corneal buttons from patients diagnosed with three different types of stromal corneal dystrophies and healthy corneas were investigated. Paraffin embedded sections were stained immunohistochemically with monoclonal antibodies against human intercellular adhesion molecule-1 (ICAM-1), endothelial selectin (Eselectin) and endothelial cadherin (E-cadherin) using the avidin-biotin-peroxidase-complex technique. The sections were compared to normal eye bank controls. In corneas from granular dystrophy patients ICAM-1 was expressed focally in epithelial cells and in keratocytes, and expressed diffusely in endothelial cells. In corneas from macular dystrophy patients diffuse epithelial staining was observed and the stromal and endothelial expression was found to be similar to that of granular dystrophy. In lattice dystrophy, only the epithelial cells and endothelium were intensively positive for ICAM-1. E-selectin was not present on any layer of the corneal specimens. E-cadherin was observed only in the epithelium of all three types of corneal dystrophies. Normal corneas did not express any of the investigated adhesion molecules. We found different expression patterns of adhesion molecules in corneas from stromal dystrophies. Our results suggest that adhesion molecules may be involved in the pathogenesis of corneal stromal dystrophies.

Original languageEnglish
Pages (from-to)945-952
Number of pages8
JournalHistology and Histopathology
Volume23
Issue number8
Publication statusPublished - Aug 2008

Fingerprint

Hereditary Corneal Dystrophies
Cell Adhesion Molecules
Cornea
Intercellular Adhesion Molecule-1
Cadherins
Epithelial Cells
Eye Banks
Selectins
E-Selectin
Avidin
Macular Degeneration
Biotin
Paraffin
Peroxidase
Endothelium
Epithelium
Endothelial Cells
Monoclonal Antibodies
Staining and Labeling

Keywords

  • Cell adhesion molecule
  • E-cadherin
  • E-selectin
  • Immunohistochemistry
  • Intercellular adhesion molecule-1
  • Stromal corneal dystrophy

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Anatomy
  • Cell Biology

Cite this

Cell adhesion molecules in stromal corneal dystrophies. / Nemeth, Gabor; Felszeghy, S.; Kenyeres, Annamaria; Szentmary, Nora; Berta, A.; Süveges, I.; Módis, L.

In: Histology and Histopathology, Vol. 23, No. 8, 08.2008, p. 945-952.

Research output: Contribution to journalArticle

Nemeth, Gabor ; Felszeghy, S. ; Kenyeres, Annamaria ; Szentmary, Nora ; Berta, A. ; Süveges, I. ; Módis, L. / Cell adhesion molecules in stromal corneal dystrophies. In: Histology and Histopathology. 2008 ; Vol. 23, No. 8. pp. 945-952.
@article{e8545163d07e4988b6520579d32e4ce6,
title = "Cell adhesion molecules in stromal corneal dystrophies",
abstract = "The aim of the present study was to investigate the expression pattern of different cell adhesion molecules in corneal stromal dystrophies. Fifteen corneal buttons from patients diagnosed with three different types of stromal corneal dystrophies and healthy corneas were investigated. Paraffin embedded sections were stained immunohistochemically with monoclonal antibodies against human intercellular adhesion molecule-1 (ICAM-1), endothelial selectin (Eselectin) and endothelial cadherin (E-cadherin) using the avidin-biotin-peroxidase-complex technique. The sections were compared to normal eye bank controls. In corneas from granular dystrophy patients ICAM-1 was expressed focally in epithelial cells and in keratocytes, and expressed diffusely in endothelial cells. In corneas from macular dystrophy patients diffuse epithelial staining was observed and the stromal and endothelial expression was found to be similar to that of granular dystrophy. In lattice dystrophy, only the epithelial cells and endothelium were intensively positive for ICAM-1. E-selectin was not present on any layer of the corneal specimens. E-cadherin was observed only in the epithelium of all three types of corneal dystrophies. Normal corneas did not express any of the investigated adhesion molecules. We found different expression patterns of adhesion molecules in corneas from stromal dystrophies. Our results suggest that adhesion molecules may be involved in the pathogenesis of corneal stromal dystrophies.",
keywords = "Cell adhesion molecule, E-cadherin, E-selectin, Immunohistochemistry, Intercellular adhesion molecule-1, Stromal corneal dystrophy",
author = "Gabor Nemeth and S. Felszeghy and Annamaria Kenyeres and Nora Szentmary and A. Berta and I. S{\"u}veges and L. M{\'o}dis",
year = "2008",
month = "8",
language = "English",
volume = "23",
pages = "945--952",
journal = "Histology and Histopathology",
issn = "0213-3911",
publisher = "Histology and Histopathology",
number = "8",

}

TY - JOUR

T1 - Cell adhesion molecules in stromal corneal dystrophies

AU - Nemeth, Gabor

AU - Felszeghy, S.

AU - Kenyeres, Annamaria

AU - Szentmary, Nora

AU - Berta, A.

AU - Süveges, I.

AU - Módis, L.

PY - 2008/8

Y1 - 2008/8

N2 - The aim of the present study was to investigate the expression pattern of different cell adhesion molecules in corneal stromal dystrophies. Fifteen corneal buttons from patients diagnosed with three different types of stromal corneal dystrophies and healthy corneas were investigated. Paraffin embedded sections were stained immunohistochemically with monoclonal antibodies against human intercellular adhesion molecule-1 (ICAM-1), endothelial selectin (Eselectin) and endothelial cadherin (E-cadherin) using the avidin-biotin-peroxidase-complex technique. The sections were compared to normal eye bank controls. In corneas from granular dystrophy patients ICAM-1 was expressed focally in epithelial cells and in keratocytes, and expressed diffusely in endothelial cells. In corneas from macular dystrophy patients diffuse epithelial staining was observed and the stromal and endothelial expression was found to be similar to that of granular dystrophy. In lattice dystrophy, only the epithelial cells and endothelium were intensively positive for ICAM-1. E-selectin was not present on any layer of the corneal specimens. E-cadherin was observed only in the epithelium of all three types of corneal dystrophies. Normal corneas did not express any of the investigated adhesion molecules. We found different expression patterns of adhesion molecules in corneas from stromal dystrophies. Our results suggest that adhesion molecules may be involved in the pathogenesis of corneal stromal dystrophies.

AB - The aim of the present study was to investigate the expression pattern of different cell adhesion molecules in corneal stromal dystrophies. Fifteen corneal buttons from patients diagnosed with three different types of stromal corneal dystrophies and healthy corneas were investigated. Paraffin embedded sections were stained immunohistochemically with monoclonal antibodies against human intercellular adhesion molecule-1 (ICAM-1), endothelial selectin (Eselectin) and endothelial cadherin (E-cadherin) using the avidin-biotin-peroxidase-complex technique. The sections were compared to normal eye bank controls. In corneas from granular dystrophy patients ICAM-1 was expressed focally in epithelial cells and in keratocytes, and expressed diffusely in endothelial cells. In corneas from macular dystrophy patients diffuse epithelial staining was observed and the stromal and endothelial expression was found to be similar to that of granular dystrophy. In lattice dystrophy, only the epithelial cells and endothelium were intensively positive for ICAM-1. E-selectin was not present on any layer of the corneal specimens. E-cadherin was observed only in the epithelium of all three types of corneal dystrophies. Normal corneas did not express any of the investigated adhesion molecules. We found different expression patterns of adhesion molecules in corneas from stromal dystrophies. Our results suggest that adhesion molecules may be involved in the pathogenesis of corneal stromal dystrophies.

KW - Cell adhesion molecule

KW - E-cadherin

KW - E-selectin

KW - Immunohistochemistry

KW - Intercellular adhesion molecule-1

KW - Stromal corneal dystrophy

UR - http://www.scopus.com/inward/record.url?scp=46249117878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46249117878&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 945

EP - 952

JO - Histology and Histopathology

JF - Histology and Histopathology

SN - 0213-3911

IS - 8

ER -