Ceftazidime–avibactam and ceftolozane–tazobactam susceptibility of multidrug resistant Pseudomonas aeruginosa strains in Hungary

Dustin O’Neall, Emese Juhasz, Akos Toth, Edit Urban, Judit Szabo, Szilvia Melegh, Katalin Katona, Katalin Kristof

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Our objective was to compare the activity ceftazidime-avibactam (C/A) and ceftolozane–tazobactam (C/T) against multidrug (including carbapenem) resistant Pseudomonas aeruginosa clinical isolates collected from six diagnostic centers in Hungary and to reveal the genetic background of their carbapenem resistance. Two hundred and fifty consecutive, non-duplicate, carbapenem-resistant multidrug resistant (MDR) P. aeruginosa isolates were collected in 2017. Minimal inhibitory concentration values of ceftazidime, cefepime, piperacillin/tazobactam, C/A and C/T were determined by broth microdilution method and gradient diffusion test. Carbapenem inactivation method (CIM) test was performed on all isolates. Carbapenemase-encoding blaVIM, blaIMP, blaKPC, blaOXA-48-like and blaNDM genes were identified by multiplex PCR. Of the isolates tested, 33.6% and 32.4% showed resistance to C/A and C/T, respectively. According to the CIM test results, 26% of the isolates were classified as carbapenemase producers. The susceptibility of P. aeruginosa isolates to C/A and C/T without carbapenemase production was 89% and 91%, respectively. Of the CIM-positive isolates, 80% were positive for blaVIM and 11% for blaNDM. The prevalence of Verona integron-encoded metallo-beta-lactamase (VIM)-type carbapenemase was 20.8%. NDM was present in 2.8% of the isolates. Although the rate of carbapenemase-producing P. aeruginosa strains is high, a negative CIM result indicates that either C/A or C/T could be effective even if carbapenem resistance has been observed.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalActa microbiologica et immunologica Hungarica
Volume67
Issue number1
DOIs
Publication statusPublished - Mar 1 2020

Keywords

  • Carbapenem resistance
  • Carbapenemase
  • Ceftazidime-avibactam
  • Ceftolozane–tazobactam
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Ceftazidime–avibactam and ceftolozane–tazobactam susceptibility of multidrug resistant Pseudomonas aeruginosa strains in Hungary'. Together they form a unique fingerprint.

  • Cite this