CE to monitor endotoxins by protein complexation

Aniko Kilár, Béla Kocsis, Ildikó Kustos, Ferenc Kilár, Stellan Hjertén

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.

Original languageEnglish
Pages (from-to)4188-4195
Number of pages8
JournalELECTROPHORESIS
Volume27
Issue number21
DOIs
Publication statusPublished - Nov 1 2006

Keywords

  • CE
  • Endotoxin
  • Hemoglobin
  • Lipopolysaccharide Protein complexation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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  • Cite this

    Kilár, A., Kocsis, B., Kustos, I., Kilár, F., & Hjertén, S. (2006). CE to monitor endotoxins by protein complexation. ELECTROPHORESIS, 27(21), 4188-4195. https://doi.org/10.1002/elps.200600276