CE to monitor endotoxins by protein complexation

Aniko Kilár, Béla Kocsis, Ildikó Kustos, F. Kilár, Stellan Hjertén

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.

Original languageEnglish
Pages (from-to)4188-4195
Number of pages8
JournalElectrophoresis
Volume27
Issue number21
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Complexation
Endotoxins
Electrophoretic mobility
Salmonella
Lipopolysaccharides
Hemoglobins
Proteins
Shigella sonnei

Keywords

  • CE
  • Endotoxin
  • Hemoglobin
  • Lipopolysaccharide Protein complexation

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Kilár, A., Kocsis, B., Kustos, I., Kilár, F., & Hjertén, S. (2006). CE to monitor endotoxins by protein complexation. Electrophoresis, 27(21), 4188-4195. https://doi.org/10.1002/elps.200600276

CE to monitor endotoxins by protein complexation. / Kilár, Aniko; Kocsis, Béla; Kustos, Ildikó; Kilár, F.; Hjertén, Stellan.

In: Electrophoresis, Vol. 27, No. 21, 11.2006, p. 4188-4195.

Research output: Contribution to journalArticle

Kilár, A, Kocsis, B, Kustos, I, Kilár, F & Hjertén, S 2006, 'CE to monitor endotoxins by protein complexation', Electrophoresis, vol. 27, no. 21, pp. 4188-4195. https://doi.org/10.1002/elps.200600276
Kilár, Aniko ; Kocsis, Béla ; Kustos, Ildikó ; Kilár, F. ; Hjertén, Stellan. / CE to monitor endotoxins by protein complexation. In: Electrophoresis. 2006 ; Vol. 27, No. 21. pp. 4188-4195.
@article{d8c233b9aa3f44b49362e975015b8023,
title = "CE to monitor endotoxins by protein complexation",
abstract = "A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between {"}smooth{"} and {"}rough{"} lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.",
keywords = "CE, Endotoxin, Hemoglobin, Lipopolysaccharide Protein complexation",
author = "Aniko Kil{\'a}r and B{\'e}la Kocsis and Ildik{\'o} Kustos and F. Kil{\'a}r and Stellan Hjert{\'e}n",
year = "2006",
month = "11",
doi = "10.1002/elps.200600276",
language = "English",
volume = "27",
pages = "4188--4195",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "21",

}

TY - JOUR

T1 - CE to monitor endotoxins by protein complexation

AU - Kilár, Aniko

AU - Kocsis, Béla

AU - Kustos, Ildikó

AU - Kilár, F.

AU - Hjertén, Stellan

PY - 2006/11

Y1 - 2006/11

N2 - A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.

AB - A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between "smooth" and "rough" lipopolysaccharides from Salmonella minnesota (wild-type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.

KW - CE

KW - Endotoxin

KW - Hemoglobin

KW - Lipopolysaccharide Protein complexation

UR - http://www.scopus.com/inward/record.url?scp=33751273612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751273612&partnerID=8YFLogxK

U2 - 10.1002/elps.200600276

DO - 10.1002/elps.200600276

M3 - Article

C2 - 17075946

AN - SCOPUS:33751273612

VL - 27

SP - 4188

EP - 4195

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 21

ER -