CDNA library preparation from a single wheat kernel

Imre Takács, Dávid Köszegi, Beáta Barnabás

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simplified cDNA cloning protocol was elaborated, easily scalable to very different sizes of plant tissue. The mRNA fraction was extracted from a single wheat kernel with oligo dT magnetic beads. The solid-phase mRNA was transcribed into single-strand cDNA by reverse transcription. Then DNA tags were incorporated into the ds cDNA fragments prior to PCR amplification. The amplified DNA was ligated to a T overhang cloning vector and some of the resulting clones were sequenced. These sequences were identified by BLASTN search in wheat EST databases.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalActa Biologica Cracoviensia Series Botanica
Volume50
Issue number1
Publication statusPublished - 2008

Keywords

  • DNA sequencing
  • EST
  • Solid-phase PCR
  • Wheat kernel
  • cDNA library

ASJC Scopus subject areas

  • Plant Science

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