CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset

Véronique Boudolf, Tim Lammens, Joanna Boruc, Jelle van Leene, Hilde van den Daele, Sara Maes, Gert van Isterdael, Eugenia Russinova, Eva Kondorosi, Erwin Witters, Geert de Jaeger, Dirk Inzé, Lieven de Veylder

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Abstract

The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.

Original languageEnglish
Pages (from-to)1482-1493
Number of pages12
JournalPlant physiology
Volume150
Issue number3
DOIs
Publication statusPublished - Jul 1 2009

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ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Boudolf, V., Lammens, T., Boruc, J., van Leene, J., van den Daele, H., Maes, S., van Isterdael, G., Russinova, E., Kondorosi, E., Witters, E., de Jaeger, G., Inzé, D., & de Veylder, L. (2009). CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset. Plant physiology, 150(3), 1482-1493. https://doi.org/10.1104/pp.109.140269