CD11c/CD18 dominates adhesion of human monocytes, macrophages and dendritic cells over CD11b/CD18

Noémi Sándor, Szilvia Lukácsi, Rita Ungai-Salánki, Norbert Orgován, B. Szabó, R. Horváth, A. Erdei, Zsuzsa Bajtay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) belong to the family of beta2 integrins and are expressed mainly by myeloid cell types in humans. Previously, we proved that CR3 rather than CR4 plays a key role in phagocytosis. Here we analysed how CD11b and CD11c participate in cell adhesion to fibrinogen, a common ligand of CR3 and CR4, employing human monocytes, monocyte-derived macrophages (MDMs) and monocyte-derived dendritic cells (MDDCs) highly expressing CD11b as well as CD11c. We determined the exact numbers of CD11b and CD11c on these cell types by a bead-based technique, and found that the ratio of CD11b/CD11c is 1.2 for MDDCs, 1.7 for MDMs and 7.1 for monocytes, suggesting that the function of CD11c is preponderant in MDDCs and less pronounced in monocytes. Applying state-of-the-art biophysical techniques, we proved that cellular adherence to fibrinogen is dominated by CD11c. Furthermore, we found that blocking CD11b significantly enhances the attachment of MDDCs and MDMs to fibrinogen, demonstrating a competition between CD11b and CD11c for this ligand. On the basis of the cell surface receptor numbers and the measured adhesion strength we set up a model, which explains the different behavior of the three cell types.

Original languageEnglish
Article numbere0163120
JournalPLoS One
Volume11
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

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Macrophages
dendritic cells
monocytes
Dendritic Cells
adhesion
Monocytes
macrophages
Adhesion
Fibrinogen
CD18 Antigens
Ligands
fibrinogen
Complement Receptors
Bond strength (materials)
Cell adhesion
Cell Surface Receptors
Myeloid Cells
cells
Phagocytosis
Cell Adhesion

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

CD11c/CD18 dominates adhesion of human monocytes, macrophages and dendritic cells over CD11b/CD18. / Sándor, Noémi; Lukácsi, Szilvia; Ungai-Salánki, Rita; Orgován, Norbert; Szabó, B.; Horváth, R.; Erdei, A.; Bajtay, Zsuzsa.

In: PLoS One, Vol. 11, No. 9, e0163120, 01.09.2016.

Research output: Contribution to journalArticle

Sándor, Noémi ; Lukácsi, Szilvia ; Ungai-Salánki, Rita ; Orgován, Norbert ; Szabó, B. ; Horváth, R. ; Erdei, A. ; Bajtay, Zsuzsa. / CD11c/CD18 dominates adhesion of human monocytes, macrophages and dendritic cells over CD11b/CD18. In: PLoS One. 2016 ; Vol. 11, No. 9.
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