CD spectrometric methods for the simultaneous determination of ethisterone and its Δ5-isomer

D. Szegvári, P. Horváth, A. Gergely, S. Németh, S. Görög

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Quick and accurate direct and indirect circular dichroism (CD) spectrometric methods were developed for the simultaneous determination of ethisterone (17α-ethinyl-17-hydroxy-4-androstene-3-one) and its Δ5-isomer (Δ5-ethisterone). The direct method is based on the selective negative Cotton effect of the Δ 4-3-oxo group in ethisterone (negative maximum at 348nm in dioxan) and measurement of the ellipticity at 296nm (positive maximum of Δ 5-ethisterone), where the measured ellipticity is the sum of those of the two isomers. In the indirect procedure Δ5-ethisterone is transformed to ethisterone by base-catalysed isomerization and the ellipticities are measured at 339nm in ethanol before and after isomerization. Preliminary experiments show the usefulness of CD detector in the HPLC determination of the mixture of the isomers. A major advantage of the direct CD spectrometric and the HPLC/CD methods is that the Δ5-isomer with extremely low UV activity can also be directly measured with high sensitivity.

Original languageEnglish
Pages (from-to)713-717
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume375
Issue number5
DOIs
Publication statusPublished - Mar 2003

Fingerprint

Ethisterone
Dichroism
Circular Dichroism
Isomers
Isomerization
Androstenes
High Pressure Liquid Chromatography
Cotton
Ethanol
Detectors

Keywords

  • Chiroptical detector
  • Circular dichroism
  • Ellipticity
  • Ethisterone
  • Isomerization

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

CD spectrometric methods for the simultaneous determination of ethisterone and its Δ5-isomer. / Szegvári, D.; Horváth, P.; Gergely, A.; Németh, S.; Görög, S.

In: Analytical and Bioanalytical Chemistry, Vol. 375, No. 5, 03.2003, p. 713-717.

Research output: Contribution to journalArticle

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