CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge

Christian Juaneda, Pierrette Lafon-Dubourg, Philippe Ciofi, Alain Sarrieau, Tibor Wenger, Gérard Tramu, Marc Corio

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The expression of cholecystokinin (CCK) mRNA in neuroendocrine corticotropin-releasing hormone (CRH) neurons of the hypothalamic paraventricular nucleus (PVN) of male rats was examined 8 h following an acute immune challenge by intraperitoneal lipopolysaccharide (LPS, 250 μg/kg). Both quantitative, macroautoradiographic, single-label radioactive in situ hybridization histochemistry (ISHH) and qualitative dual-label ISHH were performed. Compared to controls, LPS-injected rats displayed increased (185%) parvicellular CCK mRNA expression levels, occurring in a majority (70%) of CRH neurons as revealed by dual-label ISHH.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalBrain research
Issue number1-2
Publication statusPublished - May 18 2001



  • Hypothalamic paraventricular nucleus
  • Hypothalamo-pituitary-adrenal axis
  • In situ hybridization
  • Lipopolysaccharide
  • Neuropeptide

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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