CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge

Christian Juaneda, Pierrette Lafon-Dubourg, Philippe Ciofi, Alain Sarrieau, T. Wenger, Gérard Tramu, Marc Corio

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The expression of cholecystokinin (CCK) mRNA in neuroendocrine corticotropin-releasing hormone (CRH) neurons of the hypothalamic paraventricular nucleus (PVN) of male rats was examined 8 h following an acute immune challenge by intraperitoneal lipopolysaccharide (LPS, 250 μg/kg). Both quantitative, macroautoradiographic, single-label radioactive in situ hybridization histochemistry (ISHH) and qualitative dual-label ISHH were performed. Compared to controls, LPS-injected rats displayed increased (185%) parvicellular CCK mRNA expression levels, occurring in a majority (70%) of CRH neurons as revealed by dual-label ISHH.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalBrain Research
Volume901
Issue number1-2
DOIs
Publication statusPublished - May 18 2001

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Corticotropin-Releasing Hormone
Cholecystokinin
In Situ Hybridization
Neurons
Messenger RNA
Paraventricular Hypothalamic Nucleus
Lipopolysaccharides

Keywords

  • Hypothalamic paraventricular nucleus
  • Hypothalamo-pituitary-adrenal axis
  • In situ hybridization
  • Lipopolysaccharide
  • Neuropeptide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge. / Juaneda, Christian; Lafon-Dubourg, Pierrette; Ciofi, Philippe; Sarrieau, Alain; Wenger, T.; Tramu, Gérard; Corio, Marc.

In: Brain Research, Vol. 901, No. 1-2, 18.05.2001, p. 277-280.

Research output: Contribution to journalArticle

Juaneda, C, Lafon-Dubourg, P, Ciofi, P, Sarrieau, A, Wenger, T, Tramu, G & Corio, M 2001, 'CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge', Brain Research, vol. 901, no. 1-2, pp. 277-280. https://doi.org/10.1016/S0006-8993(01)02365-4
Juaneda, Christian ; Lafon-Dubourg, Pierrette ; Ciofi, Philippe ; Sarrieau, Alain ; Wenger, T. ; Tramu, Gérard ; Corio, Marc. / CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge. In: Brain Research. 2001 ; Vol. 901, No. 1-2. pp. 277-280.
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